MRNA & PEPTIDE SYNTHESIS OF VASOPRESSIN AND OXYTOCIN

信使核糖核酸

基本信息

项目摘要

The proposed experiments are designed to detect, at the level of the single cell, the alterations that follow physiologically meaningful stimulation of vasopressinergic and oxytocinergic neurons. Much is known about these neurons, but previous investigations studied global alterations in levels of mRNA and proposes. Individual neurons within apparently homogeneous anatomical nuclei differ in terms of their afferent and efferent connectivity, and in terms of peptide co-localization. Hence, in approach to neuroendocrine function should be employed tbat can detact if hormone response is restricted to specific vasopressin or oxytocin cells. Utilizing quantitative in situ hybridization to determine relative amounts of vasopressin and oxytocin mRNA levels, I will examine whether all hormone-synthesizing cells within a hypothalamic cell group response to certain physiological challenges, or whether specific subgroups of cells mediate hormone response. Based upon investigations of cell connectivity, and other factors described below, it is hypothesized that hormone response is mediated by specific vasopressinergic and oxytocinergic cells. Combining in situ hybridization with immunocytochemistry for peptide co-localization, and with tract-tracing techniques, I hope to determine the dynamic, anatomical pattern of response in vivo, of these neural systems, and the basis for elucidating the salient characteristics of those cells that specifically mediate response to homeostatic challenge. In addition to providing a model for how the function of single neurons is integrated within the context of a neuroendocrine system, these studies may have clinical relevance. First, disease processes that disrupt the regulation of synthesis, transport, or release of vasopressin can precipitate inappropriate compensation of bodily fluid needs. Proposed experiments that involve water balance and how the neurohypophysial system responds are relevant. Second, vasopressin plays a role in hypertension and stress, and study of neuronal response at the single cell level will provide information regarding differential response in autonomic or neurohaemal-mediating vasopressinergic neurons compared to neurohypophysial neurons. Finally, oxytocin gene expression is an almost unexplored matter that may be clinically relevant to oxytocin's role in pregnancy, lactation, and stress.
提出的实验旨在检测,在水平

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
An assessment of the efficacy of in situ hybridization as a quantitative method by variance components estimation.
通过方差分量估计评估原位杂交作为定量方法的功效。
  • DOI:
    10.1002/jemt.1070250109
  • 发表时间:
    1993
  • 期刊:
  • 影响因子:
    2.5
  • 作者:
    McCabe,JT;Kao,TC;Volkov,ML
  • 通讯作者:
    Volkov,ML
Quantitative in situ hybridization to measure single-cell changes in vasopressin and oxytocin mRNA levels after osmotic stimulation.
定量原位杂交可测量渗透刺激后单细胞加压素和催产素 mRNA 水平的变化。
  • DOI:
    10.1007/bf00733636
  • 发表时间:
    1990
  • 期刊:
  • 影响因子:
    4
  • 作者:
    McCabe,JT;Kawata,M;Sano,Y;Pfaff,DW;Desharnais,RA
  • 通讯作者:
    Desharnais,RA
Fos-like immunoreactivity in the brain of homozygous diabetes insipidus Brattleboro and normal Long-Evans rats.
纯合性尿崩症 Brattleboro 大鼠和正常 Long-Evans 大鼠大脑中的 Fos 样免疫反应性。
Fos and Jun expression in rat supraoptic nucleus neurons after acute vs. repeated osmotic stimulation.
急性与重复渗透刺激后大鼠视上核神经元中 Fos 和 Jun 的表达。
  • DOI:
    10.1016/s0006-8993(96)01216-4
  • 发表时间:
    1997
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Wang,K;Guldenaar,SE;McCabe,JT
  • 通讯作者:
    McCabe,JT
Detection of vasopressin mRNA in the neurointermediate lobe of the rat pituitary.
大鼠垂体神经中叶加压素 mRNA 的检测。
  • DOI:
    10.1016/0169-328x(90)90046-g
  • 发表时间:
    1990
  • 期刊:
  • 影响因子:
    0
  • 作者:
    McCabe,JT;Lehmann,E;Chastrette,N;Hänze,J;Lang,RE;Ganten,D;Pfaff,DW
  • 通讯作者:
    Pfaff,DW
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JOSEPH T MCCABE其他文献

JOSEPH T MCCABE的其他文献

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{{ truncateString('JOSEPH T MCCABE', 18)}}的其他基金

Amelioration of soman-induced neuropathology with NAAG-related compounds
用 NAAG 相关化合物改善梭曼诱导的神经病理学
  • 批准号:
    9146440
  • 财政年份:
    2015
  • 资助金额:
    $ 11.19万
  • 项目类别:
TRANSCRIPTIONAL CONTROL IN NEUROHYPOPHYSIAL NEURONS
神经垂体神经元的转录控制
  • 批准号:
    2892367
  • 财政年份:
    1998
  • 资助金额:
    $ 11.19万
  • 项目类别:
TRANSCRIPTIONAL CONTROL IN NEUROHYPOPHYSIAL NEURONS
神经垂体神经元的转录控制
  • 批准号:
    2696736
  • 财政年份:
    1998
  • 资助金额:
    $ 11.19万
  • 项目类别:
TRANSCRIPTIONAL CONTROL IN NEUROHYPOPHYSIAL NEURONS
神经垂体神经元的转录控制
  • 批准号:
    6188221
  • 财政年份:
    1998
  • 资助金额:
    $ 11.19万
  • 项目类别:
TRANSCRIPTIONAL CONTROL IN NEUROHYPOPHYSIAL NEURONS
神经垂体神经元的转录控制
  • 批准号:
    6488011
  • 财政年份:
    1998
  • 资助金额:
    $ 11.19万
  • 项目类别:
MRNA & PEPTIDE SYNTHESIS OF VASOPRESSIN AND OXYTOCIN
信使核糖核酸
  • 批准号:
    3411457
  • 财政年份:
    1989
  • 资助金额:
    $ 11.19万
  • 项目类别:
MRNA & PEPTIDE SYNTHESIS OF VASOPRESSIN AND OXYTOCIN
信使核糖核酸
  • 批准号:
    3411452
  • 财政年份:
    1989
  • 资助金额:
    $ 11.19万
  • 项目类别:
MRNA & PEPTIDE SYNTHESIS OF VASOPRESSIN AND OXYTOCIN
信使核糖核酸
  • 批准号:
    3411455
  • 财政年份:
    1989
  • 资助金额:
    $ 11.19万
  • 项目类别:
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