GENES, PROGESTINS, AND FEMALE REPRODUCTIVE BEHAVIOR

基因、孕激素和女性生殖行为

• 批准号: 3428397
• 负责人: NEAL G SIMON
• 金额: $ 2.37万
• 依托单位: LEHIGH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1988-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3428397
• 关键词: ethology; gel electrophoresis; gel filtration chromatography; gene expression; genetic regulation; hormone metabolism; progesterone; progestins; radiotracer; sex behavior

Progesterone (P) facilitates the expression of sexually receptive behaviors in non-primate female mammals and has been implicated in the etiology of cyclical mood changes in humans (premenstrual syndrome). The mechanisms through which P and its primary metabolite, 5Alpha-dihydroprogesterone (DHP), affect neural tissues and behavior, however, have not yet been completely described. Because receptor binding is recognized as a critical first step in the production of steroid-mediated effects, the goal of the proposed research will be to examine the interaction of P and DHP with cellular progestin receptors (PR) in the brain. This will be accomplished by developing an assay for neural PR in which 3H-P and 3H-DHP can be used as ligands, a procedure which is currently unavailable. The assay will then be used to compare P and DHP binding in two strains of mice (CD-1, Swiss-Websster) that are differentially sensitive to the behavioral effects of P and DHP. Since CD-1 females respond to both progestins while SW females are insensitive to DHP, the strain comparison should provide a powerful tool for characterizing a possible molecular basis for sensitivity (or insensitivity) to P and DHP as well as providing information about the induction of these receptors. The results should therefore provide insights into a major neuroendocrine process involved in cyclical behavioral changes in females and may also help generate new hypotheses concerning the possible role of progestins in premenstrual syndrome.

孕激素（P）促进性接受行为的表达 在非灵长类雌性哺乳动物中，并与以下疾病的病因有关 人类的周期性情绪变化（经前综合征）。 的机制 P及其初级代谢产物5 α-二氢孕酮 (DHP)，影响神经组织和行为，然而，还没有 完全描述。 因为受体结合被认为是 第一步，在生产类固醇介导的影响，目标的 拟议的研究将是研究P和DHP的相互作用， 脑中的细胞促甲状腺激素受体（PR）。 这将是完成 通过开发可使用3 H-P和3 H-DHP的神经PR测定法， 作为配体，这是一种目前无法获得的方法。 证明试剂盒 然后用于比较两种小鼠品系（CD-1， Swiss-Websster）对行为效应的敏感性差异 P和DHP的。 由于CD-1女性对两种孕激素都有反应，而SW 女性对DHP不敏感，菌株比较应提供 一个强大的工具，用于表征敏感性的可能分子基础 (or不敏感性），以及提供有关的信息， 诱导这些受体。 因此，结果应提供 深入了解参与周期性神经内分泌的主要神经内分泌过程， 女性行为的变化，也可能有助于产生新的假设 关于孕激素在经前综合征中的可能作用。