CONNECTIONS OF THE CHOLINERGIC BASAL FOREBRAIN

胆碱能基底前脑的连接

• 批准号: 3428488
• 负责人: ALICE S POWERS
• 金额: $ 2.01万
• 依托单位: ST. JOHN'S UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1987-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3428488
• 关键词: afferent nerve; brain mapping; brain septal area; cognition; corpus striatum; efferent nerve; histochemistry /cytochemistry; memory; prosencephalon

The present proposal is directed at understanding the connections of cholinergic cell groups in the basal telencephalon of turtles. These cell groups have recently been identified and have been shown to resemble the cholinergic cell groups of the basal forebrain of mammals in their location, histochemistry, and known projections. The basal forebrain cholinergic cell groups in mammals have been implicated in cognitive function and memory. Because the forebrains of nonmammals are considerably less complicated than those of mammals, they provide ideal preparations for studying the cholinergic system. The proposed experiments will study the connections of each of the major cholinergic cell groups: the medial septum, the ventral paleostriatum (anatomically similar to the nucleus basalis of Meynert in mammals), and the horizontal limb of the diagonal band of Broca. The research proposed here has two aims. The first is to investigate more thoroughly the anatomy of the basal forebrain systems by (a) studying the efferents of the cholinergic cell groups and (b) studying the afferents to these groups. Anterograde and retrograde tracing techniques will be employed. The second is to develop a new neuroanatomical tracing technique for use with turtles. This technique, which uses Phaseolus vulgaris leucoagglutinin (PHA-L), a kidney bean lectin, has proven useful as an anterograde tracing technique with mammals but has not yet been employed with nonmammals. Study of the simpler forebrain cholinergic system of reptiles should permit a better understanding of this system in mammals. It will also provide the foundation for behavioral studies on the function of the system in cognition and memory in reptiles.

本提案旨在了解 乌龟基底端脑内胆碱能细胞群。这些细胞 最近发现了一些群体，并显示它们类似于 哺乳动物基底前脑胆碱能细胞群 位置，组织化学，和已知的投射。基底前脑 哺乳动物中的胆碱能细胞群与认知功能有关 功能和内存。因为非哺乳动物的前脑相当于 与哺乳动物相比，它们的复杂性更低，为 研究胆碱能系统。拟议的实验将研究 各主要胆碱能细胞群的联系：内侧 隔，腹侧古纹状体(解剖上类似于核 哺乳动物的Meynert基底肌)，以及对角线的水平肢体 布罗卡乐队。 这里提出的研究有两个目的。第一是进行更多的调查 通过(A)研究基底前脑系统的解剖学 胆碱能细胞群的传出和(B)胆碱能细胞群的传入 这些团体。顺行和逆行追踪技术将是 受雇的。二是开发一种新的神经解剖示踪技术 和海龟一起使用。这项技术使用了普通菜豆 亮氨酸凝集素(PHA-L)是一种菜豆凝集素，已被证明是一种有用的 哺乳动物顺行追踪技术，但尚未应用 和非哺乳类动物。 爬行动物更简单的前脑胆碱能系统的研究应该被允许 更好地了解哺乳动物的这一系统。它还将提供 为系统功能的行为学研究奠定基础 爬行动物的认知和记忆。