ELECTROPHYSIOLOGICAL CORRELATES OF VASOPRESSIN RELEASE

其目标是了解加压素(也称为抗利尿剂)是如何 荷尔蒙)的分泌是通过电信号控制的 加压素分泌神经元的放电模式。为了实现这一目标， 这些神经元的放电模式与后叶加压素的释放有关 一种体外模型系统，大鼠视上-神经垂体系统，其中 神经活性化合物可以改变放电模式，这些化合物代表 神经递质在整个动物中对这些神经元的控制。特定的 目标是1)将组胺和去甲肾上腺素的作用联系起来 加压素释放对突发性电活动的作用 视上神经分泌神经元；2)确定是否不同 这些化合物的等位受体介导不同的作用；3)确定 这些化合物与化合物的电子状态的相互作用 神经分泌神经元在诱发突发性电活动中的作用 简明地定位这些化合物对后叶加压素和 应用细胞内标记和免疫细胞化学技术研究催产素神经元 开始表征与动作有关的膜事件 这些化合物与细胞内记录；以及6)确定 后叶加压素的突发性活性之间的定量关系 神经元和加压素的释放量和时程。这些目标 将通过运用电生理学、神经药理学、 免疫细胞化学和放射免疫分析。 这里描述的模型系统提供了一种研究 电活动与激素释放的关系。现在大家都知道 许多细胞，本质上是非神经细胞，也表现出电活动 当释放激素时，这些研究回答的问题可能 我们将适用于体内的多种细胞类型。后叶加压素 特别是在确定适当的水平衡方面是非常重要的 哺乳动物，包括人类，以及它的控制功能障碍导致许多 生理异常，包括尿崩症和慢性尿崩症 高钠血症。

The goal is to understand how vasopressin (also called antidiuretic hormone) secretion is controlled through the control of the electrical firing patterns of vasopressin-secreting neurons. To achieve this goal, firing patterns of these neurons are correlated with vasopressin release in a model in vitro system, the rat supraoptico-neurohypophysial system, where firing patterns can be altered by neuroactive compounds which represent the neurotransmitter control of these neurons in the whole animal. Specific goals are 1) to relate the actions of histamine and noradrenaline on vasopressin release to their actions on bursts of electrical activity in supraoptic neurosecretory neurons; 2) to determine if different isoreceptors of these compounds mediate different actions; 3) to determine the interaction of these compounds with the electrical state of the neurosecretory neuron in evoking bursts of electrical activity; 4) to concisely localize the effects of these compounds on vasopressin and oxytocin neurons using intracellular labelling and immunocytochemistry; 5) to begin characterization of the membrane events related to the action of these compounds with intracellular recordings; and 6) to determine the quantitative relationship between a burst of activity in a vasopressin neuron and the amount and time course of vasopressin release. These aims will be realized by employing electrophysiology, neuropharmacology, immunocytochemistry and radioimmunoassay. The model system described herein provides a means of studying the relationship of electrical activity and hormone release. It is now known that many cells, non-neuronal in nature, also exhibit electrical activity when releasing hormone, and the questions answered by these studies could well be applicable to many cell types in the body. Vasopressin in particular is very important in determining proper water balance in all mammals, including man, and dysfunctions in its control lead to many physiological irregularities, including diabetes insipidus and chronic hypernatremia.