Instrumentation for Undergraduate Lab Instruction in Molecular Cell Biology Using Non-Radioactive Labels and Computer Data Analysis

使用非放射性标记和计算机数据分析进行分子细胞生物学本科实验室教学的仪器

• 批准号: 9750703
• 负责人: Barbara Chapman
• 金额: $ 2.99万
• 依托单位: California State University San Marcos Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9750703&HistoricalAwards=false
• 关键词: Instrumentation; Undergraduate; Lab; Instruction; Molecular

Preparing undergraduates for careers in the biological sciences requires a laboratory-rich curriculum, which includes hands-on experience with current technologies. Many methods in cell and molecular biology involve exposure to toxic and radioactive chemicals. The new instrumentation is part of an effort to redesign the bioscience curriculum around nonradioactive, nontoxic methodologies. Exercises that teach quantitation of nucleic acids and proteins (including PCR products), DNA, RNA, and antibody blotting, analysis of SDS-PAGE, DNA sequencing, protease and nuclease assays, gel-shift assays for DNA-binding proteins, CAT reporter assays, and protein phosphorylation assays used in signal transduction studies can be performed with nonhazardous fluorescent labels instead of toxic or radioactive detection reagents. The new instrumentation also provides for computer data analysis, designed to improve student understanding of computational tools in biology and help them to process and report their data. Providing for direct, safer experiences with current technologies allows students to broaden and deepen their understanding of laboratory applications in biomedical science. Concerns of students, especially women students, about potential reproductive hazards should be significantly reduced. When fully implemented, the program can eliminate the need for radioisotopes and toxic dyes like ethidium bromide, along with their associated financial and environmental costs. This approach to laboratory instruction in the molecular biosciences should be broadly applicable and is particularly important for the department's attempts to increase the number of students preparing to be teachers who opt to take biology as their science and who will be better prepared in this science..

为本科生从事生物科学职业做好准备需要丰富的实验室课程，其中包括当前技术的实践经验。 细胞和分子生物学中的许多方法都涉及接触有毒和放射性化学物质。 新仪器是围绕非放射性、无毒方法重新设计生物科学课程的一部分。 教授核酸和蛋白质（包括 PCR 产物）、DNA、RNA 和抗体印迹定量、SDS-PAGE 分析、DNA 测序、蛋白酶和核酸酶测定、DNA 结合蛋白的凝胶迁移测定、CAT 报告基因测定和信号转导研究中使用的蛋白质磷酸化测定的练习可以使用无害的荧光标记而不是有毒的荧光标记进行。 或放射性检测试剂。 新仪器还提供计算机数据分析，旨在提高学生对生物学计算工具的理解，并帮助他们处理和报告数据。 利用当前技术提供直接、更安全的体验，使学生能够扩大和加深对生物医学科学实验室应用的理解。 学生，尤其是女学生对潜在生殖危害的担忧应显着减少。 全面实施后，该计划可以消除对放射性同位素和溴化乙锭等有毒染料的需求，以及相关的财务和环境成本。 这种分子生物科学实验室教学方法应该广泛适用，对于该系试图增加准备成为教师的学生数量尤其重要，这些学生选择将生物学作为他们的科学，并在这门科学上做好更好的准备。