CHARACTERIZATION OF MUSCARINIC RECEPTORS OF THE CILIARY

胆碱能机制在神经系统的生理学中起着重要的作用。 灵长类眼前节，以及青光眼的治疗。 的 建议的研究旨在增加我们对基本的 睫状肌胆碱能药理学的体外研究 从食蟹猴获得的这种肌肉的收缩反应。 这些体外研究将提供新的信息， 不能从体内研究中获得。 具体而言，这些研究将： a.确定了许多胆碱能激动剂的效力和亲和力 在睫状肌中 B.定义该系统中许多毒蕈碱拮抗剂的pA2 C.确定睫状肌是否是“备用受体”系统 D.确定纤毛中是否存在多种毒蕈碱亚型 肌肉 e. 确定受体结合是否受鸟嘌呤核苷酸调节 本提案中的体内研究将建立剂量反应 醋克立定是一种胆碱能激动剂，据报道， 眼内压，对调节的影响最小。 这些研究应该提高我们对胆碱能神经的作用的理解。 使用自主神经药物降低眼内压的机制 开角型青光眼的治疗。

Cholinergic mechanisms play an important role in the physiology of the primate anterior ocular segment, as well as in treatment of glaucoma. The studies proposed are designed to increase our understanding of the basic cholinergic pharmacology of the ciliary muscle by investigating in vitro the contractile responses of this muscle obtained from cynomolgus monkeys. These in vitro studies will provide information that is novel and that is not obtainable from studies in vivo. Specifically, these studies will: a. define the potencies and affinities for a number of cholinergic agonists in the ciliary muscle b. define the pA2 for a number of muscarinic antagonists in this system c. determine whether or not the ciliary muscle is a "spare receptor" system d. determine whether there are multiple muscarinic subtypes in the ciliary muscle e. determine whether receptor binding is modulated by guanine nucleotides In vivo studies in this proposal will establish a dose response relationship for aceclidine, a cholinergic agonist that reportedly lowers intraocular pressure with minimal effects on accommodation. These studies should improve our understanding of the role of cholinergic mechanisms when autonomic drugs are used to reduce the intraocular pressure in treatment of open-angle glaucoma.