CHARACTERIZATION OF MUSCARINIC RECEPTORS OF THE CILIARY

胆碱能机制在心力衰竭的生理学中起重要作用。 灵长类眼前节，以及青光眼的治疗。这个 建议进行的研究旨在加深我们对 睫状肌胆碱能药理作用的体外研究 食蟹猴肌肉的收缩反应。 这些体外研究将提供新的信息，即 不能从活体研究中获得。具体地说，这些研究将： A.定义一些胆碱能激动剂的效力和亲和力 在睫状肌中 B.定义该系统中多个M受体拮抗剂的PA2 C.确定睫状肌是否为“备用感受器”系统 D.确定睫状肌中是否存在多种毒鼠碱亚型 肌肉 即确定受体结合是否受鸟嘌呤核苷酸的调节 这项建议中的体内研究将建立一个剂量反应 乙酰胆碱，一种胆碱能激动剂，据报道降低 眼内压对调节的影响最小。 这些研究应该会提高我们对胆碱能作用的理解。 自主神经药物降低眼压的作用机制 治疗开角型青光眼。

Cholinergic mechanisms play an important role in the physiology of the primate anterior ocular segment, as well as in treatment of glaucoma. The studies proposed are designed to increase our understanding of the basic cholinergic pharmacology of the ciliary muscle by investigating in vitro the contractile responses of this muscle obtained from cynomolgus monkeys. These in vitro studies will provide information that is novel and that is not obtainable from studies in vivo. Specifically, these studies will: a. define the potencies and affinities for a number of cholinergic agonists in the ciliary muscle b. define the pA2 for a number of muscarinic antagonists in this system c. determine whether or not the ciliary muscle is a "spare receptor" system d. determine whether there are multiple muscarinic subtypes in the ciliary muscle e. determine whether receptor binding is modulated by guanine nucleotides In vivo studies in this proposal will establish a dose response relationship for aceclidine, a cholinergic agonist that reportedly lowers intraocular pressure with minimal effects on accommodation. These studies should improve our understanding of the role of cholinergic mechanisms when autonomic drugs are used to reduce the intraocular pressure in treatment of open-angle glaucoma.