PHARMACOLOGY OF AQUEOUS OUTFLOW IN THE EYE

眼房水流出的药理学

• 批准号: 2161438
• 负责人: KRISTINE A ERICKSON
• 金额: $ 24.32万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2161438
• 关键词: Animalia; adrenergic agents; anterior chamber; biological models; cholinergic agents; cyclic AMP; drug receptors; epinephrine; eye pharmacology; glaucoma; human tissue; intraocular aqueous flow; ocular hypotensive; perfusion; phosphorylation; physiology; protein kinase; receptor sensitivity; sclera; second messengers; trabecular meshwork

DESCRIPTION (Investigator's Abstract): The overall goal of proposed research is to gain further knowledge of the physiology and pharmacology of aqueous outflow in the human eye.In proposed studies, the investigators have chosen to look at two general classes of drugs used therapeutically in the treatment of glaucoma; adrenergic agents and cholinergic agents. The specific aims are to characterize outflow facility responses to these drugs in her recently described human isolated outflow pathway perfusion model, to map the distribution of drug receptors in outflow tissues, and to characterize relevant second messenger systems. This three-pronged approach, coordinating studies in physiology, biochemistry, and molecular biology, has the potential for producing a wealth of information greater than the sum of each individual approach in isolation. Recent refinements in molecular biology techniques and their applications is revolutionizing the diagnosis and treatment of disease, as well as basic medical research. The investigator's hope that her approach, using this powerful new technology in conjunction with the human TM model will allow her to develop a composite map of the human outflow system which will localize groups of cells which mediate changes in outflow resistance and characterize the second messenger systems which are coupled to these changes. Enhanced understanding of the basic mechanism(s) underlying normal outflow physiology may lend new insight into the pathogenesis of primary open angle glaucoma, and probably will facilitate the development of more effective, specific, and safe antiglaucoma drugs.

描述（研究者的摘要）：提议的总体目标 研究的目的是为了获得更多的生理学和药理学知识 人眼中的房水流出。在拟议的研究中，研究人员 选择研究两种一般类别的治疗药物 青光眼的治疗；肾上腺素能药物和胆碱能药物。 这 具体目标是描述流出设施对这些药物的反应 在她最近描述的人体隔离流出通路灌注模型中， 绘制流出组织中药物受体的分布图，并 描述相关第二信使系统的特征。这个三管齐下 方法，协调生理学、生物化学和分子研究 生物学具有产生大量信息的潜力 而不是孤立地使用每个单独方法的总和。 最近的改进 分子生物学技术及其应用正在发生革命性的变化 疾病的诊断和治疗以及基础医学研究。 调查员希望她的方法能够利用这种强大的新方法 技术与人类 TM 模型相结合将使她能够开发 人口流出系统的综合地图，将定位人群 介导流出阻力变化并表征的细胞 与这些变化耦合的第二信使系统。 增强型 了解正常流出的基本机制 生理学可能为原发性开角的发病机制提供新的见解 青光眼，并且可能会促进更有效的开发， 特异性、安全的抗青光眼药物。