CONTROL OF CELL MIGRATION IN DEVELOPING BRAIN

这项研究的目的是为了更好地了解 引导年轻神经元离开增殖区的一系列事件 它在成熟大脑中的适当位置，才能开始理解 特定的基因可能有助于(和/或破坏)迁徙过程， 并分析处于错误位置的神经元的能力 在差异化的同时调整他们的连接性。该方法 将利用小鼠体内存在的突变进行研究 扰乱了发育中的中枢神经中神经元的迁移 系统，从而产生成人大脑畸形，其中 特定的神经元群体位于不正常的位置。特定的 本项目的目标是：1)确定开发流程 被影响位置的四种不同的遗传缺陷所修饰 最终由年轻的神经元在从 增殖带到它们在发展过程中的最终位置 中枢神经系统；2)分析成熟中枢神经系统 神经元轴突和树突分支的变化 在发育期间迁移到错误的位置。 将进行的实验将：1)探索相关的 迁移神经元本身的贡献，放射状胶质细胞的贡献， 先前产生的神经元和邻近结构的轴突在 影响幼鱼迁徙轨迹的“停止”点 神经元，2)分析神经元之间的形态差异 达到“错误”的立场和那些已经采取“正确”立场的人 立场，以及3)澄清实验的遗传学的一些方面 情况。这项工作意义重大，因为它对 对中枢神经系统发育中细胞迁移的认识 神经系统。

The goals of this research are to reach a better understanding of the sequence of events which lead a young neuron from the proliferative zones to its proper position in the mature brain, to begin to understand how specific genes might contribute to (and/or disrupt) the migratory process, and to analyze the capacity of neurons which are in the wrong position to make adjustments in their connectivity while differentiating. The approach to be taken will be to exploit the existence of mutations in the mouse which disrupt the migration of neurons in the developing central nervous system and thereby produce malformations of the adult brain in which specific populations of neurons reside in abnormal positions. Specific aims of this project are: 1) to determine the development processes modified by four different genetic defects which affect the position ultimately assumed by young neurons during their migration from the proliferative zone to their final position during the development of the central nervous system, and 2) to analyze in the mature central nervous system the changes in the axonal and dendritic arborizations of neurons which migrate to the wrong position during the developmental period. Experiments will be conducted which will: 1) explore the relative contributions of the migrating neurons themselves, of radial glial cells, of previously generated neurons, and of axons from adjacent structures in influencing the "stopping" point along the migratory trajectory of a young neuron, 2) analyze the morphological differences between neurons which have reached a "wrong" position and those which have assumed the "correct" position, and 3) clarify some aspects of the genetics of the experimental situations. This work is significant because of its contribution to the understanding of cell migration during the development of the central nervous system.