REGULATION OF SYNAPTIC SPECIFICITY

建议继续进行轴突再生的研究。 以及神经细胞间突触形成的特异性。一个 对这些现象和相关现象的详细了解将在 扩展关于正常和异常的知识必不可少 发展，并制定帮助患者康复的策略 神经系统损伤后的功能。这一制度 在原代细胞培养中可识别的大型海兔神经元将 用于检测突触的特异性和突触的可塑性。 这些神经元再生神经突起并形成特定的电信号。 和化学突触在培养中，已经发现 生长模式和特异性可以通过添加 凝集素刀豆球蛋白A加入培养基中。因此，虽然 刀豆蛋白A本身不太可能参与神经元 开发，它可以作为一种工具来研究基础 突触发生中的问题。现在建议在这些基础上 发现并询问有关分子机制的问题 刀豆蛋白A的作用，使用 电生理、生化和分子生物学 接近了。这些多学科研究的长期目标是 是为了了解突触特异性的分子基础和 突触可塑性，这是正常的 神经系统的功能。

It is proposed to continue ongoing studies of neurite regeneration and specificity of synapse formation between nerve cells. A detailed understanding of these and related phenomena will be essential for extending knowledge about normal and abnormal development, and for devising strategies to help patients recover function following injury to the nervous system. The system of large identifiable Aplysia neurons in primary cell culture will be used to examine synaptic specificity and synaptic plasticity. These neurons regenerate neurites and form specific electrical and chemical synapses in culture, and it has been found that the patterns of growth and specificity can be changed by adding the lectin Concanavalin A to the culture medium. Thus, although Concanavalin A itself is unlikely to be involved in neuronal development, it can be used as a tool to investigate fundamental problems in synaptogenesis. It is proposed now to build on these findings and ask questions about the molecular mechanisms of action of Concanavalin A, using a combination of electrophysiological, biochemical and molecular biological approaches. The long term goal of these multidisciplinary studies is to understand the molecular basis of synaptic specificity and synaptic plasticity, phenomena which are essential for the proper functioning of the nervous system.