High-throughput Optical Blood Imaging for the Detection of Rare Cells using Ultrasonic Particle Alignment

使用超声波颗粒对准检测稀有细胞的高通量光学血液成像

• 批准号: EP/K027115/1
• 负责人: Peter Glynne-Jones
• 金额: $ 12万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FK027115%2F1
• 关键词: throughput; Optical; Blood; Imaging; Detection

Millions of blood samples are analysed in the NHS every year. A wide range of parameters are measured, however there are significant limitations with existing technologies, particularly those relating to the detection of rare cells and bacteria in the blood in a way that permits timely medical intervention.Modern computers have the power to examine images of billions of blood cells, and flag up those of interest. However, taking images of the cells individually (as is done in some existing machines) is not fast enough, being limited to a few thousand cells per second. In this project we will flow a sample of blood through a tiny chamber and use ultrasound to align all the cells into a single sheet; this key step means that it will be possible for a microscope to take an image of thousands of the cells at once. In this way we predict it will be possible to image around 50,000 cells per second. Without this acoustic focussing, the cells would not all be in focus, and many would overlap with each other. Working with our partners at Southampton hospital, we shall initially test our system by looking for cancer cells in the blood. Recent research has shown that individual cells from a cancer can become detached and enter the blood stream, and it is in this way that cancers spread to different areas of the body (metastasize). These cells are present in very small numbers - in 10 ml of blood even one tumour cell hidden amongst billions of normal cells could indicate a cancer. Later on in the project we will also consider looking at leukaemia cells, parasite infections (such as malaria), and potentially bacterial infections.There are several challenges facing the project including creating an ultrasonic force field that is both strong and uniformly focussed, the difficulties of capturing images of the moving stream of blood cells without motion blur, and integrating the image processing to deal with the huge amounts of data that will be produced. The different applications we consider vary enormously in the challenge they present, ranging from the tiny and difficult to manipulate bacteria, through to the much more numerous malaria parasites inside red blood cells.Given the huge potential health benefits of having our systems made widely available, we will work closely with industrial companies that have experience of creating diagnostic machines to bring our technology into widespread use as soon as possible. Into the future we see many further developments, including systems that are able to capture the rare cells as they are detected, and collaborative research projects with clinical scientists to use our devices to enable new insights into the workings of diseases.

NHS每年要分析数百万份血液样本。测量的参数范围很广，但现有技术存在重大局限性，特别是在检测血液中的稀有细胞和细菌以便及时进行医疗干预方面。现代计算机有能力检查数十亿血细胞的图像，并标记出感兴趣的那些。然而，单独拍摄细胞的图像（就像在一些现有的机器上所做的那样）不够快，每秒只能拍摄几千个细胞。在这个项目中，我们将血液样本流过一个小室，并使用超声波将所有细胞排列成一张单片；这一关键步骤意味着显微镜将有可能一次拍摄数千个细胞的图像。通过这种方式，我们预测每秒将有可能成像大约5万个细胞。如果没有这种声学聚焦，细胞就不会全部聚焦，而且许多细胞会相互重叠。我们将与南安普顿医院的合作伙伴合作，首先通过寻找血液中的癌细胞来测试我们的系统。最近的研究表明，来自癌症的单个细胞可以分离并进入血液，正是通过这种方式，癌症扩散到身体的不同部位（转移）。这些细胞的数量非常少——在10毫升血液中，即使是隐藏在数十亿正常细胞中的一个肿瘤细胞也可能表明癌症。在项目的后期，我们还将考虑研究白血病细胞、寄生虫感染（如疟疾）和潜在的细菌感染。该项目面临着几个挑战，包括创建一个既强又均匀聚焦的超声波力场，在没有运动模糊的情况下捕获移动的血细胞流图像的困难，以及整合图像处理以处理将产生的大量数据。我们考虑的不同应用在它们所带来的挑战上差异很大，从微小且难以操纵的细菌，到红细胞内数量更多的疟疾寄生虫。鉴于我们的系统广泛应用对健康有巨大的潜在好处，我们将与有制造诊断机器经验的工业公司密切合作，使我们的技术尽快得到广泛应用。展望未来，我们看到了许多进一步的发展，包括能够捕获被检测到的稀有细胞的系统，以及与临床科学家合作的研究项目，利用我们的设备对疾病的运作产生新的见解。

项目成果

Acoustofluidic phase microscopy in a tilted segmentation-free configuration.

• DOI: 10.1063/5.0036585
• 发表时间: 2021-01
• 期刊: Biomicrofluidics
• 影响因子: 3.2
• 作者: J. Mejía Morales;B. Hammarström;G. Lippi;M. Vassalli;P. Glynne-Jones

Application of an acoustofluidic perfusion bioreactor for cartilage tissue engineering.

• DOI: 10.1039/c4lc00956h
• 发表时间: 2014-12-07
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Li S;Glynne-Jones P;Andriotis OG;Ching KY;Jonnalagadda US;Oreffo RO;Hill M;Tare RS
• 通讯作者: Tare RS

High throughput imaging cytometer with acoustic focussing.

高通量成像细胞仪，具有声音聚焦。

• DOI: 10.1039/c5ra19497k
• 发表时间: 2015-10-31
• 期刊: RSC advances
• 影响因子: 3.9
• 作者: Zmijan R;Jonnalagadda US;Carugo D;Kochi Y;Lemm E;Packham G;Hill M;Glynne-Jones P
• 通讯作者: Glynne-Jones P

Acoustic focussing for sedimentation-free high-throughput imaging of microalgae

• DOI: 10.1007/s10811-019-01907-5
• 发表时间: 2019-09
• 期刊: Journal of Applied Phycology
• 影响因子: 3.3
• 作者: B. Hammarström;M. Vassalli;P. Glynne-Jones