IN VITRO INVASION MODEL FOR PERIODONTOPATHOGENS

牙齿支持组织的感染包括多种 临床上离散的实体并归入人类牙周 疾病。 在这些疾病中，牙周炎是最常见的，也是最常见的 当牙龈炎症蔓延到结缔组织时就会出现这种情况 组织附着和牙槽骨支撑牙齿。 微生物 与牙周病的病因学有关。 超过 人类口腔内存在 300 种细菌，但其中只有少数 这些被认为与牙周病有关。 最接近的 牙周感染与微生物之间存在关联 局限性幼年性牙周炎与放线杆菌之间的关系 伴放线菌。 A. actinomycetemcomitans 也涉及 某些类型的成人牙周炎。 任何细菌的精确作用 牙周病的种类尚不清楚。 许多研究人员指出， 然而，重要的周围病原体能够渗透 上皮细胞。 我们假设细菌入侵电话和 组织在牙周病的发病机制中发挥着重要作用。 我们的 近期目标是开发 A 的体外入侵模型。 利用培养的哺乳动物细胞的放线菌。 一些 将筛选 A. actinomycetemcomitans 菌株以确定 入侵频率的差异。 我们的长期目标是 细菌和宿主细胞在入侵中的作用 过程。

Infections of the supporting tissues of the teeth include a number of clinically discrete entities and are grouped under human periodontal diseases. Of these diseases, periodontitis is the most common and results when inflammation in the gingiva is extended to the connective tissue attachment and alveolar bone supporting teeth. Microorganisms have been implicated in the etiology of periodontal disease. More than 300 bacterial species reside in the human oral cavity, but only a few of these are believed to be involved in periodontal disease. The closest association between a periodontal infection and an organism exists between Localized Juvenile Periodontitis and Actinobacillus actinomycetemcomitans. A. actinomycetemcomitans is also implicated in certain types of adult periodontitis. The precise role of any bacterial species in periodontal disease is not known. Many investigators noted, however, that the significant periopathogens are able to penetrate epithelial cells. We hypothesize that bacterial invasion of calls and tissues plays a role in the pathogenesis of periodontal disease. Our immediate goal is to develop an in vitro invasion model for A. actinomycetemcomitans utilizing cultured mammalian cells. A number of A. actinomycetemcomitans strains will be screened to determine differences in invasion frequencies. Our long term goal is to look at the role of both the bacterium and the host cell in the invasion process.