GENOTOXICITY OF GLUTARALDEHYDE--SAFE USE IN DENTISTRY

戊二醛的遗传毒性——在牙科中的安全使用

• 批准号: 3425560
• 负责人: ANN J DUNIPACE
• 金额: $ 2.24万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425560
• 关键词: aldehydes; antiseptic sterilization; chromosome aberrations; cytogenetics; dental bonding material; dentin; dentistry; drug adverse effect; frameshift mutation; glutarates; mutagen testing; mutagens; tissue /cell culture

Glutaraldehydel, (5-pentanedial) is a bifunctional aldehyde which is currently being used in dentistry as a pulpotomy medicament, dentin bonding mediator, sterilizing agent, and for various tissue preparation procedures. Its two, highly reactive terminal aldehyde groups rapidly and irreversibly react with proteins while reversibly reacting with DNA. Glutaraldehyde is used at a concentration of at least 2% in all of the dental applications mentioned. Toxicity of the compound in laboratory animals is unclear due to conflicting information, but strong irritation and skin sensitization have been reported. Irritation to the eyes, lungs, skin and gastric mucosa have been reported in humans. Mutagenicity information on glutaraldehyde is limited and available results are contradictory. Only one in vivo study has been reported. Therefore, the purpose of this study is to investigate the genotoxic potential of glutaraldehyde using five independent tests. These tests include: (1) the Ames Salmonella test; (2) an in vitro Chinese hamster ovary cell sister chromatid exchange assay; (3) an a in vivo Chinese hamster bone marrow cell sister chromatid exchange assay; (4) a mouse sperm morphology test; and (5) a mouse bone marrow micronucleus study. Because each test has strengths and weaknesses, a battery of carefully-selected in vitro and in vivo test systems will be used to evaluate the mutagenic potential of glutaraldehyde. It is believed that this series of genotoxic tests, taken collectively, will provide reliable data on the genetic effects, if any, of glutaraldehyde, and will help establish the risks or safety of its continued use in dentistry.

戊二醛（5-戊二醛）是一种双功能醛