PROPHYLAXIS OF L. TROPICA INFECTIONS WITH CYCLOSPORINES

用环孢菌素预防热带乳杆菌感染

• 批准号: 3436589
• 负责人: NANCY C BEHFOROUZ
• 金额: $ 6.43万
• 依托单位: BALL STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1988-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3436589
• 关键词: Leishmania; cell population study; cyclosporines; disease /disorder model; immunosuppression; leishmaniasis; leukocyte activation /transformation; lymphokines; protozoal antigen

The proposed study on the prophylaxis of Leishmania tropica infections in Balb/c mice with the known immunosuppressant Cyclosporine A and its analogue, B-5-49, will attempt to elucidate the drug-induced alteration in the host response to the parasite which enables this highly susceptible mouse strain to mount an effective curative immune response to the infecting protozoa. Leishmania tropica infections in Balb/c mice have been used as experimental models of both diffuse cutaneous and the frequently fatal systemic leishmaniasis in man. Our understanding, treatment and prevention of these two world-wide infections is strikingly incomplete or inadequate. It is hoped that this study will help to clarify the important immunomodulatory effects of the two cyclosporines which apparently prevents leishmania specific immunosuppression from developing during the infectious process. After determining the optimum protective treatment regimen for both drugs, the antigen-specific and non-specific immune responsiveness of infected, treated animals will be monitored and compared to non-treated controls. Specifically, the development and level of: 1) delayed type hypersensitivity, 2) anti-leishmanial antibodies and 3) resistance to parasite challenge will be determined over time as well as level of immune responsiveness in vitro in response to unrelated antigens (SRBC) and mitogens. Of particular interest will be the ability of lymphocytes from the experimental animals to produce lymphokines which are capable of stimulating macrophage cidal activity toward intracellular leishmania. The level of immunosuppression in treated, non-infected animals will also be determined to clearly establish the level of immunocompetence following the chosen treatment regimen for both drugs.

预防热带利什曼原虫感染的初步研究

项目成果

Prophylactic treatment of BALB/c mice with cyclosporine A and its analog B-5-49 enhances resistance to Leishmania major.

用环孢素 A 及其类似物 B-5-49 对 BALB/c 小鼠进行预防性治疗可增强对重大利什曼原虫的抵抗力。

• 发表时间: 1986
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Behforouz,NC;Wenger,CD;Mathison,BA
• 通讯作者: Mathison,BA

Immunomodulation of murine leishmaniasis with cyclosporin A.

用环孢菌素 A 对小鼠利什曼病进行免疫调节。

• DOI: 10.1007/978-1-4757-5421-6_36
• 发表时间: 1988
• 期刊: Advances in experimental medicine and biology
• 作者: Behforouz,NC;Wenger,CD
• 通讯作者: Wenger,CD