ESTROGEN-INDUCED NORMAL AND DYSPLASTIC UTERINE GROWTH
ESTROGEN-INDUCED NORMAL AND DYSPLASTIC UTERINE GROWTH
批准号:
3426707
负责人:
WILLIAM J HENDRY
金额:
$4.97万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-05-01 至 1993-04-30
关键词:
cell type cheek pouch technique diethylstilbestrol epithelium estrogens gene expression hamsters histogenesis histology homologous transplantation hormone regulation /control mechanism hormone related neoplasm /cancer hormone therapy hyperplasia implant messenger RNA neoplastic transformation newborn animals northern blottings ovariectomy protooncogene reproductive tissue transplantation stromal cells tissue /cell culture uterus uterus preneoplastic state
中文摘要
当仓鼠在出生时接受合成雌激素治疗时,
英文摘要
When hamsters are treated neonatally with the synthetic estrogen,
diethylstilbestrol (DES), their uteri consistently exhibit a severe
hyperplastic/neoplastic response to estrogen in adulthood. One of two
alternative working hypotheses should explain this phenomenon: 1) Direct
Action - The cellular physiology and/or composition of the neonatal
hamster uterus is directly and permanently altered by the DES insult such
that the adult organ's overall proliferative response to estrogen becomes
atypical, or 2) Indirect Action - Uterotrophic activity is mediated by
estrogen primarily through or in conjunction with other unidentified
factors, and neonatal DES treatment permanently alters the level or
functional activity of such factors. Testing these hypotheses will begin
(Specific Aim # 1) by monitoring the morphogenesis of neonatal uteri from
control and DES-treated donor animals that are transplanted into the
contralateral cheek pouches of control and DES-treated mature hosts that
are either left intact, estrogen-deprived or estrogen-replaced. To
confirm and extend the findings (Specific Aim #2), homotypic and
heterotypic recombination of uterine stroma and epithelium from control
and DES-treated animals will be performed and their morphogenesis will be
studied: a) in vitro using media supplemented with uterine tissue
extracts and/or serum from the same host groups used in Specific Aim # 1
and b) in vivo (within the cheek pouch, using the same host groups as in
Specific Aim #l. Lastly (Specific Aim #3), Northern blot analysis will
be used to consider the possibility that altered expression of two
proto-oncogenes (c-myc and c-fos) may be involved in the atypical
estrogen responsiveness of uteri in neonatally DES-exposed hamsters.
Because this combination of approaches relies on few, if any, a priori
assumptions and accommodates both in vivo and in vitro observations, firm
conclusions should be reached about which alternative hypothesis is most
valid. Such information should contribute significantly to the long-term
objective of understanding how estrogen regulates uterine growth and
morphogenesis. This objective is biomedically important because: 1)
successful conception and gestation demands normal uterine form and
function, and 2) estrogen-dependent uterine neoplasms are responsible for
considerable morbidity and mortality in contemporary American society.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Wichita State University Combined Core Facility Renovation project
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批准号:7935973
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项目类别:
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资助金额:$219.1万
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财政年份:2010
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负责人:WILLIAM J HENDRY
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依托单位:
UTERINE DISRUPTION: DNA METHYLATION & EPITHELIA-STROMA
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批准号:6875775
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项目类别:
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资助金额:$14.28万
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财政年份:2003
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负责人:WILLIAM J HENDRY
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依托单位:
UTERINE DISRUPTION: DNA METHYLATION & EPITHELIA-STROMA
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批准号:6648181
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项目类别:
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资助金额:$14.3万
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财政年份:2003
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负责人:WILLIAM J HENDRY
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依托单位:
UTERINE DISRUPTION: DNA METHYLATION & EPITHELIA-STROMA
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批准号:6740254
-
项目类别:
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资助金额:$14.29万
-
财政年份:2003
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负责人:WILLIAM J HENDRY
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依托单位:
MOLECULAR TARGETS OF PERINATAL ENDOCRINE DISRUPTION
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批准号:2881611
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项目类别:
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资助金额:$10.72万
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财政年份:1999
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负责人:WILLIAM J HENDRY
-
依托单位:
SMALL INSTRUMENTATION GRANT
-
批准号:3523749
-
项目类别:
-
资助金额:$0.5万
-
财政年份:1993
-
负责人:WILLIAM J HENDRY
-
依托单位:
NEONATAL DES INDUCED UTERINE DYSPLASIA/NEOPLASIA
-
批准号:2100950
-
项目类别:
-
资助金额:$15.11万
-
财政年份:1992
-
负责人:WILLIAM J HENDRY
-
依托单位:
NEONATAL DES INDUCED UTERINE DYSPLASIA/NEOPLASIA
-
批准号:2100951
-
项目类别:
-
资助金额:$15.24万
-
财政年份:1992
-
负责人:WILLIAM J HENDRY
-
依托单位:
NEONATAL DES-INDUCED UTERINE DYSPLASIA/NEOPLASIA
-
批准号:3203946
-
项目类别:
-
资助金额:$14.59万
-
财政年份:1992
-
负责人:WILLIAM J HENDRY
-
依托单位:
NEONATAL DES-INDUCED UTERINE DYSPLASIA/NEOPLASIA
-
批准号:3203945
-
项目类别:
-
资助金额:$10.42万
-
财政年份:1992
-
负责人:WILLIAM J HENDRY
-
依托单位:
ESTROGEN-INDUCED NORMAL AND DYSPLASTIC UTERINE GROWTH
-
批准号:3426706
-
项目类别:
-
资助金额:$3.17万
-
财政年份:1991
-
负责人:WILLIAM J HENDRY
-
依托单位:
ESTROGEN-INDUCED NORMAL AND DYSPLASTIC UTERINE GROWTH
-
批准号:3426708
-
项目类别:
-
资助金额:$1.55万
-
财政年份:1991
-
负责人:WILLIAM J HENDRY
-
依托单位:
MANIPULATING GLUCOCORTICOID RECEPTOR IN LIVE TUMOR CELLS
-
批准号:3237129
-
项目类别:
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资助金额:$9.09万
-
财政年份:1987
-
负责人:WILLIAM J HENDRY
-
依托单位:
MANIPULATING GLUCOCORTICOID RECEPTOR IN LIVE TUMOR CELLS
-
批准号:3237131
-
项目类别:
-
资助金额:$9.44万
-
财政年份:1987
-
负责人:WILLIAM J HENDRY
-
依托单位:
MANIPULATING GLUCOCORTICOID RECEPTOR IN LIVE TUMOR CELLS
-
批准号:3237132
-
项目类别:
-
资助金额:$9.24万
-
财政年份:1987
-
负责人:WILLIAM J HENDRY
-
依托单位: