NEW METHODS FOR HPLC OPTICAL RESOLUTION AND DETECTION

生理特性和治疗效果的差异 许多药物的对映体形式已经被认识了一段时间。 非常 通常，不需要的对映体可以逆转或以其他方式限制该效应。 所需的对映异构体。 光学纯度知识和能力 为了常规地进行手性物质的光学拆分， 因此，对药理学家，生物学家， 生物化学家有机合成化学家动力学家 不幸的是，目前可用的光学分辨率方法不能 用于一般的手性物质，因为它们都遭受严重的 限制和约束。 这项研究的长期目标是开发一种新的 该方法简单快速，但对一般 光学纯度的常规测定和光学异构体的分离 异构体 开发的方法将基于高性能 液相色谱技术（HPLC）由于其效率、速度 适用性广。 将采取创新办法， 将临界现象引入胶束技术中， 第一次，胆盐胶束溶液在临界温度下， 手性移动的相。 这些新的手性移动的相有望 提供更高的分辨率和效率， 对一般手性物质的HPLC-光学拆分（HPLC-OR）。 的 激光、偏振和光热效应的优点将在 首次开发出激光光热折射 圆二色性分光偏振计（PR-CD），其能够 在非常低的温度下测定小体积样品的圆二色性 浓度的 该PR-CD将被用作快速，实时和 HPLC-CD超灵敏圆二色谱检测器。

Differences between physiological properties and therapeutic effects of enantiomeric forms of many drugs have been recognized for sometime. Very often, an unwanted enantiomer can reverse or otherwise limit the effect of the desired enantiomer. Knowledge on the optical purity and ability to routinely perform the optical resolution of chiral substances are, therefore, of extreme importance to pharmacologists, biologists, biochemists as well as organic synthetic chemists, kineticists... Unfortunately, the currently available optical resolution methods can not be used for general chiral substances because they all suffer severe limitations and restrictions. The long term objective of the proposed research is to develop a novel method which is easy and fast but sensitive and effective for the general and routine determination of optical purity and separation of optical isomers. The developed method will be based on the high performance liquid chromatography technique (HPLC) because of its efficiency, speed and wide applicability. An innovative approach will be taken by incorporating critical phenomena into micellar technology to develop for, the first time, bile salt micellar solutions at critical temperature as chiral mobile phases. These novel chiral mobile phases are expected to provide higher resolution and efficiency and to widen the application of HPLC-optical resolution (HPLC-OR) to the general chiral substances. The advantages of laser, polarization and the photothermal effect will be taken to develop, for the first time, a laser photothermal refraction - circular dichroism spectropolarimeter (PR-CD) which is capable of determining the circular dichroism of small volume samples at very low concentrations. This PR-CD will then be used as fast, realtime and ultrasensitive circular dichroism detector for HPLC-CD.