NEW METHODS FOR HPLC OPTICAL RESOLUTION AND DETECTION

血瘀证生理特性及疗效的差异 许多药物的对映体形式已经被认识到一段时间了。非常 通常情况下，不需要的对映体可以逆转或以其他方式限制效果。 所需的对映体。关于光学纯度和能力的知识 常规地执行手性物质的光学拆分是， 因此，对药剂学家、生物学家来说， 生物化学家以及有机合成化学家和运动学家。 遗憾的是，目前可用的光学分辨方法不能 用于一般的手性物质，因为它们都受到严重的 限制和限制。 拟议研究的长期目标是开发一种新的 一种简便、快速、灵敏、有效的一般方法 光学纯度和光学分离的常规测定 异构体。所开发的方法将基于高性能 高效、快速的高效液相色谱技术 和广泛的适用性。将采取一种创新的方法 将临界现象融入到胶束技术中来开发， 首次将临界温度下的胆盐胶束溶液 手性流动相。这些新颖的手性流动相有望 提供更高的分辨率和效率，并扩大应用 对一般手性物质的高效液相-光学拆分(HPLC-OR)。这个 激光、偏振和光热效应的优点将是 首次开发出激光光热折射-- 圆二色光谱偏振仪(PR-CD) 小体积样品极低圆二向色性的测定 浓度。这张PR-CD将被用作快速、实时和 超灵敏圆二色检测器用于高效液相色谱检测。

Differences between physiological properties and therapeutic effects of enantiomeric forms of many drugs have been recognized for sometime. Very often, an unwanted enantiomer can reverse or otherwise limit the effect of the desired enantiomer. Knowledge on the optical purity and ability to routinely perform the optical resolution of chiral substances are, therefore, of extreme importance to pharmacologists, biologists, biochemists as well as organic synthetic chemists, kineticists... Unfortunately, the currently available optical resolution methods can not be used for general chiral substances because they all suffer severe limitations and restrictions. The long term objective of the proposed research is to develop a novel method which is easy and fast but sensitive and effective for the general and routine determination of optical purity and separation of optical isomers. The developed method will be based on the high performance liquid chromatography technique (HPLC) because of its efficiency, speed and wide applicability. An innovative approach will be taken by incorporating critical phenomena into micellar technology to develop for, the first time, bile salt micellar solutions at critical temperature as chiral mobile phases. These novel chiral mobile phases are expected to provide higher resolution and efficiency and to widen the application of HPLC-optical resolution (HPLC-OR) to the general chiral substances. The advantages of laser, polarization and the photothermal effect will be taken to develop, for the first time, a laser photothermal refraction - circular dichroism spectropolarimeter (PR-CD) which is capable of determining the circular dichroism of small volume samples at very low concentrations. This PR-CD will then be used as fast, realtime and ultrasensitive circular dichroism detector for HPLC-CD.