BIOANALYTICAL APPLICATIONS OF ACOUSTO-OPTIC DEVICES

描述(改编自申请人的摘要)：本报告的目的 建议是开发新的测量仪器，这是不可能的 使用现有的技术。声光材料的独特性质 可调谐滤光器(AOTF)将被开发。具体地说，就是这种能力 用于产生多于一个波长的衍射光的AOTF 以不同频率调制的每个波长将 与热透镜技术协同结合，制造出 多波长红外热透镜毛细管检测器 几乎为零背景、高灵敏度、 和普遍性。此外，探测器将能够 通过样品的红外生色团识别样品中的官能团。 该检测器对于大分子的测序可能特别有用 比如DNA。一种灵敏、通用的高效液相和毛细管电泳手性检测器 将通过将热透镜效应与AOTF相结合来开发 测量振动圆二向色性。一种用于UV和可见光的AOTF 将生成的区域用于后续的快速扫描构建 高效液相色谱吸收圆二色检测器。优势 最近开发的集成AOTF的一部分将被带到 使仪器小型化，并为其提供附加功能 例如更快的扫描能力和消除重新对准。最后， AOTF对来自不同位置光的绕射能力 不同波长的物体将被利用来制造 凝胶和毛细管阵列电泳仪(GE和CAE) 具有真正的超多光谱成像能力(即，它能够 以空间分辨率记录对象的每个点的光谱 其分辨率可与光谱分辨率相媲美)。因为这件事 与其他探测器相比，探测器具有高灵敏度和更高的吞吐量， 这可能为DNA测序提供重大突破。总而言之， 所获得的结果将为科学家提供必要的工具 可以方便地用于灵敏、快速和(化学和 立体化学)以确定化学和生化样品(例如， 致癌化合物、药物、DNA、蛋白质) 高效液相色谱、毛细管电泳法和GE法。这些工具对人类的贡献 基因组计划将是无价的，因为它们将提供大量的 GE和CAE技术的产量增加。

DESCRIPTION (Adapted from applicant's abstract): The objective of this proposal is to develop novel instruments for measurements not possible using existing techniques. The unique properties of acousto-optic tunable filters (AOTFs) are to be exploited. Specifically, the ability of the AOTF to give diffracted light at more than one wavelength with each wavelength modulated at a different frequency will be synergistically combined with the thermal lens technique to make a multiwavelength infrared thermal lens detector for capillary electrophoresis (CE) with virtually zero background, high sensitivity, and universality. Additionally, the detector will be capable of identifying functional groups in a sample through their IR chromophores. The detector may be particularly useful for sequencing of macromolecules such as DNA. A sensitive and universal chiral detector for HPLC and CE will be developed by combining the thermal lens effect with the AOTF to measure vibrational circular dichroism. An AOTF for the UV and visible regions will be made for subsequent construction of a rapid scanning absorption and circular dichroism (CD) detector for HPLC. Advantages of the recently developed integrated-AOTF will then be taken to miniaturize the instruments and to provide them with additional features such as faster scan capability and elimination of realignment. Finally, the AOTF's ability to diffract light coming from different positions of an object with different wavelengths will be exploited to make a detector for gel and capillary array electrophoresis (GE and CAE) that has true supermultispectral imaging capability (i.e., it is capable of recording spectra for each point of an object with spatial resolution so high that it is comparable to the spectral resolution). Because this detector has high sensitivity and higher throughput the other detectors, it may provide a major breakthrough for DNA sequencing. Collectively, results obtained will provide the necessary instruments that scientists can easily use to detect sensitively and rapidly and (chemically and stereochemically) to determine chemical and biochemical samples (e.g., carcinogenic compounds, drugs, DNA, proteins) as they are separated by HPLC, CE, and GE. The contribution of these instruments to the human genome project will be invaluable because they will provide substantial increases in the throughputs of the GE and CAE techniques.