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EXERCISE, AGING, AND SULFOCONJUGATED CATECHOLAMINES

运动、衰老和磺基儿茶酚胺

基本信息

批准号：
3436400
负责人：
Mark Sothmann
金额：
$ 10.59万
依托单位：
UNIVERSITY OF WISCONSIN MILWAUKEE
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-07-01 至 1992-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3436400
关键词：
aging cardiovascular stress test catecholamines dopamine epinephrine exercise human middle age (35-64) human population dynamics neuroendocrine system radioimmunoassay sulfation sulfotransferase young adult human (21-34)

项目摘要

This research examines the impact of aging on plasma free and sulfconjugated catecholsmines at rest and during exercise stress. Furthermore, the impact of exercise training in those measures will be determined. Sulfoconjugation is an important deactivation mechanism for free catecholamines (CA) released from the sympathetic nervous system and adrenal gland. Low sulfoconjuration of free CA has been linked to essential hypertension. Advancing chronological age is characterized by augmented plasma free CA concentrations at rest and during physical exertion, due to heightened appearance rate and reduced clearanced. To date no study has examined whether the reduced clearance of CA in older individuals is due in part to low sulfoconjugation relative to free amine appearance rates. To address this issue, 20 healthy males aged 30-40 yrs and 20 healthy males aged 55-65 yrs will be examined at rest and during 15 min. of submaximal exercise (70% V02 max). The age groups will be further categorized into high-fit (45-55 ml.kg-1 min-1) and moderate-fit (25-35 ml.kg-1 min-1). Plasma free and sulfoconjugated CA profiles will be examined determine any age effect and any age x cardiovascular fitness interaction. Additionally, 10 experimental males (aged 55-65 yrs) will undergo a 12 wk exercise training program and be compared to controls to identify adaptations occurring with free CA, their sulfoconjugates, and the ratio of free to sulfocnonjugated CA. This research has importance in terms of 1) determining whether reduced sulfoconjugation is one mechanism by which clearance of CA is compromised with advancing chronological age, 2) identifying whether the postulated low sulfoconjugation ability with advanced age is attenuated by maintaining a high level of cardiovascular fitness, and 3) examining whether an intervention program designed to increase cardiovascular fitness and resulting in chronically enhanced perfusion of tissue high in phenosulfotransferase activity stimulates reduced free plasma CA with increased sulfoconjugation, thus reflecting a more desireable ratio between the active and inactive forms. This research provides further understanding of neuroendocrine processes with advancing chronological age, and offers insight into the potential importance of cardiovascular fitness and exercise training for maintaining neuroendocrine functioning.

这项研究探讨了衰老对无血浆和在休息和运动应激期间的磺基共轭儿茶酚胺。此外，运动训练在这些措施中的影响将被确定。磺基共轭是一种重要的失活作用释放的游离儿茶酚胺（CA）的机制交感神经系统和肾上腺。低磺基共轭游离CA与原发性高血压有关。推进实足年龄的特征是血浆游离CA增加休息时和体力活动时的浓度，由于提高了出现率，减少了间隙。迄今没有一项研究检查了老年人CA清除率的降低是否个体中的硫基结合部分是由于相对于游离的硫基结合低，胺出现率。为了解决这个问题，20名健康男性年龄30-40岁，20名健康男性，年龄55-65岁在休息和15分钟次极量运动期间（70%V02 max）。年龄组将进一步分为高适应性（45-55 ml.kg-1 min-1）和中度拟合（25-35 ml.kg-1 min-1）。血浆游离和磺基共轭CA配置文件将被检查，以确定任何年龄影响和任何年龄x心血管健康的相互作用。此外，将对10名实验男性（年龄55-65岁）进行 12周的运动训练计划，并与对照组进行比较，鉴定游离CA、其磺基缀合物以及游离CA与磺基非共轭CA的比率。本研究（1）确定是否减少磺基共轭是CA清除的一种机制，随着年龄的增长而妥协，2）识别假设的低磺基共轭能力与先进的通过维持高水平的心血管疾病，健身，以及3）检查是否设计了干预计划增加心血管健康，酚磺基转移酶活性高的组织的增强灌注刺激减少的游离血浆CA与增加的磺基缀合，从而反映了活性物质和不活跃的形式。这项研究提供了进一步的了解神经内分泌过程随着年龄的增长，深入了解心血管疾病的潜在重要性，维持神经内分泌的健身和运动训练功能