REGULATION OF PYRUVATE KINASE IN DROSOPHILA MELANOGASTER

果蝇丙酮酸激酶的调控

• 批准号: 3438805
• 负责人: GLEN E. COLLIER
• 金额: $ 10.29万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3438805
• 关键词: Drosophilidae; complementary DNA; cytogenetics; developmental genetics; ecdysone; gel electrophoresis; gene expression; genetic regulation; genetic transcription; genetic translation; genome; hormone regulation /control mechanism; in situ hybridization; metamorphosis; molecular cloning; molecular genetics; nucleic acid probes; nucleic acid sequence; pyruvate kinase

At the end of larval development in Drosophila melanogaster a rise in the titer of ecdysone directly stimulates the expression of a small number of genes (early genes). The protein products of these genes are responsible for the subsequent stimulation of a much larger number of genes (late genes) whose expression is necessary for normal metamorphosis. Three of these early genes, 2B5, 74EF, and 75B, have been analyzed in some detail. The identity of specific late genes and their regulatory relationships to specific early genes are poorly known. These relationships determine the coordinate and tissue specific expression of the late genes. Two enzymes (arginine kinase and pyruvate kinase) in D. melanogaster have been shown to be hormonally regulated during the prepupal period of development and this developmental regulation is influenced by the 75B early gene. To determine the molecular mechanisms responsible for this coordinate regulation requires that the genes responsible for these enzymes be cloned and characterized. The gene for arginine kinase has been isolated under other support. This proposal outlines the isolation and initial characterization of the gene from Drosophila melanogaster for pyruvate kinase.

在黑腹果蝇幼虫发育末期， 蜕皮激素滴度的升高直接刺激了 少数基因（早期基因）。 的蛋白质产物 这些基因负责随后的刺激 大量的基因（晚期基因），其表达是 是正常变态所必需的 其中三个早期基因， 2B5、74EF和75B，已经进行了一些详细的分析。 的 特异性晚期基因及其调控 与特定早期基因的关系知之甚少。 这些 关系确定坐标和组织特异性 晚期基因的表达。 两种酶（精氨酸激酶和 丙酮酸激酶）。黑腹动物已经被证明 在发育的前蛹期由昆虫调节的 这种发育调控受75B早期 基因 为了确定造成这种情况的分子机制 协调调节需要负责 这些酶被克隆和表征。 精氨酸的基因 激酶已在其他支持下分离。 这项建议 概述了该基因的分离和初步特征 从果蝇中提取丙酮酸激酶