APOFERRITIN AS A FERROXIDASE
APOFERRITIN AS A FERROXIDASE
批准号:
3437812
负责人:
RODNEY F BOYER
金额:
$6.73万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-07-15 至 1990-06-30
中文摘要
最近人们发现,铁蛋白能储存铁
英文摘要
It has recently been discovered that ferritin, the iron storage
protein, is able to catalyze the oxidation of Fe(II) to Fe(III). Some
preliminary measurements have been made of this ferroxidase
activity, but a more complete characterization of the dynamic
action of apoferritin is essential. Furthermore, it is suggested
that this observed enzymatic activity represents the first step in
ferritin iron incorporation. The research plan outlined here is
directed toward an extensive investigation of the ferroxidase
activity of apoferritin and its possible role in iron incorporation.
The specific aims are: (1) to characterize the kinetic and
molecular properties of the ferroxidation process (kinetic
parameters, substrate specificity, inhibition, amino acid
modification). This will be achieved primarily by using a
procedure for capture of the product, Fe(III), by the iron-binding
protein, transferrin; (2) to investigate aspects of the molecular
mechanism of the ferroxidation precess (stoichiometry, O2 and
other electron acceptors as oxidants, fate of O2 reduction,
identity of cofactor(s)); and (3) a comparison of the
characteristics of apoferritin as a ferroxidase with its function
iron incorporation.
In spite of numerous investigations, little is known about the role
of ferritin iron storage and utilization. Ferritin has been
implicated as a key molecule in iron metabolism and the
concentration of serum ferritin is currently used in clinical
situations to monitor iron levels/utilization and disease
conditions, including iron deficiency anaemia, solid tumors,
leukemia, and heavy metal toxicity. However, before more
clinical use is made of ferritin, we must have a greater
understanding of its dynamic role in iron metabolism. Results
from the proposed research will provide new insight into this
problem.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
Selective adsorption of apoferritin on immobilized Fe(III): demonstration of Fe(III) binding sites.
脱铁铁蛋白在固定化 Fe(III) 上的选择性吸附:Fe(III) 结合位点的演示。
DOI:
--
发表时间:
1990
期刊:
Biotechnology and applied biochemistry
影响因子:
2.8
作者:
[Boyer,RF, Generous,SM, Nieuwenhuis,TJ, Ettinger,RA]
通讯作者:
Ettinger,RA
海外基金