CHARACTERIZATION OF STREPTOCOCCUS SANGUIS IGA PROTEASE

免疫球蛋白A(IgA)是一种主要的免疫球蛋白。 人体粘膜表面。免疫球蛋白具有广泛的抗体活性 一系列细菌、病毒、毒素和酶。在人类口述中 空洞，这种活性被IgA-的存在所削弱- 血链球菌分泌的特异性蛋白酶，一种微小的 牙菌斑中突出的有机体。这样做的具体目的是 建议是：1)鉴定所产生的IgA蛋白酶；2) 确定粘性放线菌(A)的作用机制 发现与血链球菌密切相关的牙周病原体) 在生长过程中加速IgA蛋白酶的分泌过程 与链球菌共培养；3)分离鉴定 唾液中稳定IgA蛋白水解酶的物质 抗热变性和蛋白水解性；4)比较IgA 高产菌株和野生型菌株产酶的研究 血缘沙门氏菌这项研究的长期目标是：1) 开发一种化学定义的生产IgA蛋白酶的系统 这将促进酶的纯化，通过提供 较高的初始比活度，提高了分辨率 消除交叉反应介质的免疫化学研究 成分，并允许比较更高的产量 高产菌株产酶及其稳定性的研究 防止热变性和蛋白水解性，从而提供 足够的量来执行酶 鉴定和氨基酸序列研究。

Immunoglobulin A (IgA) is the predominant immunoglobulin on human mucosal surfaces. IgA has antibody activity against a wide range of bacteria, viruses, toxins and enzymes. In the human oral cavity, this activity is diminished by the presence of an IgA- specifc protease secreted by Streptococcus sanguis, a micro- organism prominent in dental plaque. The specific aims of this proposal are: 1) to characterize the IgA protease produced; 2) to determine the mechanisms by which Actinomyces viscosus (a periodontal pathogen found in close association with S. sanguis) accelerates the course of IgA protease secretion when growth in co-culture with the streptococcus; 3) to isolate and characterize the substances in human saliva which stabilize the IgA protease against thermal and proteolytic denaturation; 4) to compare IgA protease production in hyperproducer strains and wild type strains of S. sanguis. The long-term objectives of this research are: 1) to develop a chemically defined system for IgA protease production which will facilitate purification of the enzyme by providing higher initial specific activity, increase the resolution of immunochemical studies by elimination of cross-reactive media constituents, and allow comparison of higher production of the enzyme by hyperproducing strains and stabilization of the enzyme against thermal and proteolytic denaturation so as to provide sufficient quantities on which to perform enzyme characterization and amino acid sequencing studies.