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A NOVEL INSTRUMENT FOR IN VIVO CELL-TISSUE ELECTROFUSION

体内细胞组织电融合的新型仪器

基本信息

批准号：
3431449
负责人：
ROBERT J GRASSO
金额：
$ 3.04万
依托单位：
UNIVERSITY OF SOUTH FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-12-01 至 1988-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3431449
关键词：
biotechnology cell fusion disease /disorder model electric field fluorescent dye /probe photomicrography

项目摘要

Research productivity in many different biomedical disciplines will increase significantly when novel "custom made" animal models routinely become available. Hence, the long term objectives of this proposal include the creation of new laboratory models that have biological properties fundamentally different from their respective naturally occurring animal species. These unique models could be produced by directly electrofusing individual cells having particular properties to intact tissue of anesthetized animals with the aid of a newly designed and constructed scientific instrument. Such an instrument can become a reality after a number of important parameters have been characterized. In this regard, the following hypothesis is presented. "A defined set of combined electromechanical parameters exist or the optimal rapid, efficient electrofusion of individual cells to intact tissue in vivo". The specific aims in this proposal are limited in scope to testing this hypothesis as follows: 1) to examine the necessity of AC dielectrophoresis for cell-tissue alignment in vivo under defined mechanical pressures and DC electrical fusion fields; 2) to explore the role of DC fusion fields in vivo under defined mechanical pressures and AC dielectrophoretic fields (if required); 3) to integrate the most effective combined electromechanical conditions for optimizing in vivo cell-tissue electrofusion; and 4) to construct a prototype instrument having unique probes whose design and operation are based upon optimal electromechanical parameters as defined by experimentation. Newly developed instrumentation will allow us to achieve our long term objectives of creating novel animal models for accelerating biomedical research productivity in many different areas. These areas include, but are not limited to, immunology, infectious diseases, experimental therapeutics, cancer research, ophthalmology, embryology, and possibly even wound research and gene therapy.

许多不同生物医学学科的研究生产力当新的“定制”动物模型通常变得可用。因此，从长远来看，这一建议的目标包括建立新的实验室这些模型的生物学特性从它们各自的自然发生的动物物种。这些独特的模型可以通过直接电熔具有特定性质的单个细胞与完整组织的麻醉动物的帮助下，一个新设计的，建造科学仪器。这种工具可以成为现实后，一些重要的参数，被定性。在这方面，以下假设是提出了 “存在一组定义的组合机电参数，或单个细胞的最佳快速有效电融合，体内完整组织”。本提案的具体目标仅限于测试范围本文的假设如下：1）考察AC的必要性介电电泳用于在限定的条件下的体内细胞-组织对齐机械压力和直流电聚变场; 2）探索DC融合场在定义的条件下体内的作用机械压力和AC介电泳场（如果（3）以最有效的方式结合用于优化体内细胞-组织的机电条件电融合;以及4）构建原型仪器，所述原型仪器具有独特的探头，其设计和操作基于最佳的机电参数由实验定义。新开发的仪器将使我们能够实现我们的长期目标。建立新的动物模型，生物医学研究在许多不同领域的生产力。这些领域包括但不限于免疫学、感染学、疾病，实验疗法，癌症研究，眼科，胚胎学，甚至可能是伤口研究，基因治疗