A NOVEL INSTRUMENT FOR IN VIVO CELL-TISSUE ELECTROFUSION

许多不同生物医学学科的研究效率 将显著增加，当新奇的“定制”动物 机型通常都会上市。因此，从长远来看， 这项提议的目标包括创建新的实验室 具有根本不同生物学特性的模型 来自它们各自的自然产生的动物物种。这些 通过直接电熔化可以生产出独特的型号 单个细胞对完整的组织具有特殊的性质 麻醉动物在一种新设计和 建造的科学仪器。这样的仪器可以 成为现实后，一些重要的参数 被刻画出来了。在这方面，以下假设是 呈上了。 “存在一组定义的组合机电参数或 单个细胞的最佳快速、高效电融合 活体内完整的组织。 该提案中的具体目标仅限于测试 这一假设如下：1)考察交流的必要性 限定条件下体内细胞-组织排列的介电泳法 机械压力和直流电融合场；2)至 探讨DC融合场在体内的作用 机械压力和交流介质电泳场(IF 必填)；3)整合最有效的组合 优化体内细胞组织的机电条件 电融合；以及4)构建具有以下特性的样机 独特的探头，其设计和操作基于最优 由实验定义的机电参数。 新开发的仪器将使我们能够实现我们的长期 创造新型加速动物模型的学期目标 许多不同领域的生物医学研究生产力。这些 领域包括但不限于免疫学、传染性 疾病，实验治疗学，癌症研究， 眼科，胚胎学，甚至伤口研究和 基因疗法。

Research productivity in many different biomedical disciplines will increase significantly when novel "custom made" animal models routinely become available. Hence, the long term objectives of this proposal include the creation of new laboratory models that have biological properties fundamentally different from their respective naturally occurring animal species. These unique models could be produced by directly electrofusing individual cells having particular properties to intact tissue of anesthetized animals with the aid of a newly designed and constructed scientific instrument. Such an instrument can become a reality after a number of important parameters have been characterized. In this regard, the following hypothesis is presented. "A defined set of combined electromechanical parameters exist or the optimal rapid, efficient electrofusion of individual cells to intact tissue in vivo". The specific aims in this proposal are limited in scope to testing this hypothesis as follows: 1) to examine the necessity of AC dielectrophoresis for cell-tissue alignment in vivo under defined mechanical pressures and DC electrical fusion fields; 2) to explore the role of DC fusion fields in vivo under defined mechanical pressures and AC dielectrophoretic fields (if required); 3) to integrate the most effective combined electromechanical conditions for optimizing in vivo cell-tissue electrofusion; and 4) to construct a prototype instrument having unique probes whose design and operation are based upon optimal electromechanical parameters as defined by experimentation. Newly developed instrumentation will allow us to achieve our long term objectives of creating novel animal models for accelerating biomedical research productivity in many different areas. These areas include, but are not limited to, immunology, infectious diseases, experimental therapeutics, cancer research, ophthalmology, embryology, and possibly even wound research and gene therapy.