INHIBITION OF A B-CELL LYMPHOMA BY SUPPRESSOR T-CELLS

抑制性 T 细胞对 B 细胞淋巴瘤的抑制

• 批准号: 3437282
• 负责人: DAVID S CHI
• 金额: $ 5.86万
• 依托单位: EAST TENNESSEE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1989-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3437282
• 关键词: B lymphocyte; avian leukosis /sarcoma; hypogammaglobulinemia; immunosuppression; interleukin 2; leukocyte activation /transformation; lymphosarcoma; neoplasm /cancer immunotherapy; suppressor T lymphocyte; tissue /cell culture

The purpose of this study is to examine adoptive immunotherapy to induced B-cell neoplasia. Utilizing the agammaglobulinemic (A-gamma) chicken model developed in my laboratory, we will characterize suppressor T-cells induced in this model system and elucidate the suppression mechanism. An important aspect of this study will be to investigate the possibility that these suppressor T-cells may suppress not only normal B-cells but also lymphoma B-cells which are present in some avian leukoses. Suppressor cells specific for the inhibition of Ig production will be induced in A-gamma chickens (line 151/5 x 7/1) by injection of bursa cells. Suppressor cells will be characterized with respect to: 1) surface receptors, 2) adherence to nylon wool and lectins (Vicia villosa and Helix pomatia), 3) sensitivity to rabbit anti-chicken T-cells + C and radiation, and 4) other fractionation properties (density gradients on Percoll and rosetting technique). An in vitro assay for suppressor T-cells has been developed using either sheep RBC- or TNP-poly-acrylamide-primed spleen cells as targets. The mechanism of action of suppressor T-cells will be distinguished by whether they can suppress the Ig production of antigen-primed B-cells, inhibit the proliferation of mitogen-stimulation B-cells, or lyse B-cells. Suppressor T-cells obtained from spleens of bursa-immunized A-gamma chickens will be used in an attempt to suppress the growth of a B-cell leukosis line (LSCC-RP9) in vitro. The in vivo suppression of the growth of transplantable B-cell leukosis (LSCT-RP6) by suppressor T-cells will also be studied by directly transplanting LSCT-RP6 into A-gamma chickens. If the leukosis grows less in A-gamma than in normal chickens, it will indicated that the tumor may be susceptible to suppression. Further study of suppression of leukosis will be carried out by co-transfer of LSVT-RP6 cells and suppressor T-cells into normal chickens. Suppressor T-cells will also be used to treat B-cell leukosis-bearing chickens. The suppression of the growth of avian B-cell lymphomas by suppressor T-cells will potentiate a new approach for adoptive immunotherapy of B-cell neoplasms.

本研究的目的是检查过继免疫治疗诱导的 B细胞瘤 利用无丙种球蛋白血症（A-γ）鸡模型 在我的实验室开发，我们将表征抑制T细胞诱导 在此模型系统中，并阐明抑制机制。 一个重要 这项研究的一个方面将是调查的可能性，这些 抑制性T细胞不仅可以抑制正常B细胞，还可以抑制淋巴瘤 存在于某些禽类白血病中的B细胞。 抑制细胞 特异性抑制IG产生的抗体将在A-γ 鸡（品系151/5 × 7/1）通过注射囊细胞。 抑制细胞 将在以下方面进行表征：1）表面受体，2）粘附 对尼龙毛和凝集素（毛野豌豆和苹果蔓）的敏感性 兔抗鸡T细胞+ C和辐射，以及4）其他 分级性质（Percoll和玫瑰花结上的密度梯度 技术）。 一种抑制性T细胞的体外检测方法已经建立， 使用绵羊RBC-或TNP-聚丙烯酰胺-致敏的脾细胞作为 目标的 抑制性T细胞的作用机制将是 区别在于它们是否可以抑制IG的产生， 抗原引发的B细胞，抑制有丝分裂原刺激的增殖 B细胞或裂解B细胞。 抑制性T细胞，获自 将使用囊免疫的A-γ鸡试图抑制 B细胞白血病细胞系（LSCC-RP 9）的体外生长。 体内 抑制可移植B细胞白血病（LSCT-RP 6）的生长， 还将通过直接移植LSCT-RP 6来研究抑制性T细胞 变成了A-伽马鸡 如果白细胞增生在A-γ中比在 正常鸡，这将表明肿瘤可能是易感的， 镇压 对抑制白血病的研究还有待进一步深入 通过将LSVT-RP 6细胞和抑制性T细胞共转移到正常 鸡 抑制性T细胞也将用于治疗B细胞 患有白血病的鸡 抑制禽类B细胞生长 淋巴瘤的抑制性T细胞将加强一种新的方法， B细胞肿瘤的免疫治疗。

项目成果

Inhibition of a chicken B-cell lymphoma by suppressor T-cells from agammaglobulinemic chickens.

无丙种球蛋白血症鸡的抑制性 T 细胞对鸡 B 细胞淋巴瘤的抑制作用。

• 发表时间: 1990
• 期刊: Anticancer research
• 影响因子: 2
• 作者: Chi,DS;Sharma,JM
• 通讯作者: Sharma,JM