INHIBITION OF A B-CELL LYMPHOMA BY SUPPRESSOR T-CELLS

本研究的目的是检测过继免疫治疗对诱导 B细胞瘤。利用无丙种球蛋白血症(A-伽马)鸡模型 在我的实验室开发的，我们将表征诱导的抑制性T细胞 并在此模型系统中阐明了抑制机制。一个重要的 这项研究的一个方面将是调查这些 抑制性T细胞不仅可以抑制正常的B细胞，还可以抑制淋巴瘤 B细胞存在于某些禽类白血球病中。抑制细胞 特异性地抑制免疫球蛋白的产生将在A-伽马中诱导 鸡(系151/5×7/1)注射法氏囊细胞。抑制细胞 将从以下方面进行表征：1)表面受体，2)粘附性 对尼龙毛和凝集素(毛豆和毛豆)，3)敏感 对兔抗鸡T细胞C和辐射，以及4)其他 分馏性质(Percoll和玫瑰花结上的密度梯度 技术)。建立了一种抑制T细胞的体外检测方法 用绵羊RBC或TNP-聚丙烯酰胺诱导的脾细胞作为 目标。抑制性T细胞的作用机制将是 区别在于他们是否能抑制免疫球蛋白的产生 抗原激活的B细胞抑制有丝分裂原刺激的增殖 B细胞，或溶解B细胞。从小鼠脾组织中获得抑制性T细胞 法氏囊免疫的A-伽马鸡将被用来试图抑制 B细胞白血病细胞系(LSCC-RP9)的体外生长活体内 丹参对可移植性B细胞白血病生长的抑制作用 抑制性T细胞也将通过直接移植LSCT-RP6进行研究 变成A级伽马鸡。如果白血病在A-伽马射线中的生长速度低于在 正常鸡，则表明肿瘤可能易感。 压制。抑制白血病的进一步研究将被开展 LSVT-RP6细胞与抑制性T细胞共转移至正常人 小鸡。抑制性T细胞也将用于治疗B细胞 患有白血病的鸡。抑制禽类B细胞的生长 抑制性T细胞淋巴瘤将促进一种新的采用方法 B细胞肿瘤的免疫治疗。

The purpose of this study is to examine adoptive immunotherapy to induced B-cell neoplasia. Utilizing the agammaglobulinemic (A-gamma) chicken model developed in my laboratory, we will characterize suppressor T-cells induced in this model system and elucidate the suppression mechanism. An important aspect of this study will be to investigate the possibility that these suppressor T-cells may suppress not only normal B-cells but also lymphoma B-cells which are present in some avian leukoses. Suppressor cells specific for the inhibition of Ig production will be induced in A-gamma chickens (line 151/5 x 7/1) by injection of bursa cells. Suppressor cells will be characterized with respect to: 1) surface receptors, 2) adherence to nylon wool and lectins (Vicia villosa and Helix pomatia), 3) sensitivity to rabbit anti-chicken T-cells + C and radiation, and 4) other fractionation properties (density gradients on Percoll and rosetting technique). An in vitro assay for suppressor T-cells has been developed using either sheep RBC- or TNP-poly-acrylamide-primed spleen cells as targets. The mechanism of action of suppressor T-cells will be distinguished by whether they can suppress the Ig production of antigen-primed B-cells, inhibit the proliferation of mitogen-stimulation B-cells, or lyse B-cells. Suppressor T-cells obtained from spleens of bursa-immunized A-gamma chickens will be used in an attempt to suppress the growth of a B-cell leukosis line (LSCC-RP9) in vitro. The in vivo suppression of the growth of transplantable B-cell leukosis (LSCT-RP6) by suppressor T-cells will also be studied by directly transplanting LSCT-RP6 into A-gamma chickens. If the leukosis grows less in A-gamma than in normal chickens, it will indicated that the tumor may be susceptible to suppression. Further study of suppression of leukosis will be carried out by co-transfer of LSVT-RP6 cells and suppressor T-cells into normal chickens. Suppressor T-cells will also be used to treat B-cell leukosis-bearing chickens. The suppression of the growth of avian B-cell lymphomas by suppressor T-cells will potentiate a new approach for adoptive immunotherapy of B-cell neoplasms.