MATHEMATICAL MODEL FOR REPLICATION OF R1 PLASMIDS

质体是染色体外的、自主复制的遗传元件 它们被广泛用作克隆外源DNA的载体， 复制控制的研究。 拟议研究的主要目标 将是一个数学模型的复制控制的发展 R1质粒。 该模型将基于分子的序列 这些事件被认为启动了一轮质粒复制。 该模型将复制控制的多个方面集成为一个 完整模型 该模型将被测试其准确的能力， 模拟复制过程并预测当前可用的 实验观察。 该模型将用于测试 关于生物化学机制的各种假说的可接受性 参与质粒拷贝数的调节，和/或提示 一个假设是合理的条件。 质粒的模型将被纳入一个结构良好的数学模型， 大肠杆菌的单细胞模型。 这种包容性将使 对各种生长条件下拷贝数的先验预测 以及调节元件中的各种突变。 该模型还可以 用于探索宿主-质粒相互作用和各种 亚细胞机制对整个系统的反应-反应，可以 可以定量测量。 需要实验来估计模型参数或 验证模型预测。 将进行实验以获得 的浓度的增长率依赖性的信息， 监管成分。

Plasmids are extrachromosomal, autonomously replicating genetic elements which are used extensively as vehicles for cloning foreign DNA and the study of replication control. The main objective of the proposed research will be the development of a mathematical model for the replication control of R1 plasmids. The model will be based on the sequence of molecular events which are thought to initiate a round of plasmid replication. The model will integrate many facets of replication control into one complete model. The model will be tested for its ability to accurately simulate the replication process and to predict the current available experimental observation. The model will then be used to test the plausibility of various hypotheses concerning the biochemical mechanisms involved in the regulation of plasmid copy number, and/or to suggest the conditions under which a hypothesis is plausible. The model for plasmid will be included into a well structured mathematical model of a single-cell of Esherichia coli. Such an inclusion will allow the a priori predictions of copy number for a variety of growth conditions and for various mutations in the regulatory elements. The model also can be used to explore the host-plasmid interactions and the effects of various subcellular mechanisms on the overall system response - a response that can be quantitatively measured. Experiments are required either for estimation of model parameters or for verification of model predictions. Experiments will be performed to obtain information on the growth rate dependence of the concentration of the regulatory components.