MEMBRANE PROTEIN STRUCTURE

这里提出的项目的中心目标是加速 膜蛋白结构研究的进展 传播关于各种生物物理的信息 已成功应用于膜的技术 蛋白质研究。我们为此提出的具体方法 信息传播是我们提议举办的一个研讨会 1990年10月13日至18日，在范德比尔特大学校园举行 大学医学院。本研讨会遵循两个 关于膜蛋白结晶的美国学派 1985年12月在科罗拉多州博尔德举行，1987年10月， 在阿贡国家实验室，以及关于 “相互作用的实验和理论方面 确定蛋白质构象“会议于1989年1月在 美国国立卫生研究院。就像第一次和第二次 关于膜蛋白结晶的美国学派 已经选择了研讨会的形式，以便参与者可以获得 对讨论中的方法有实际操作经验。就像 然而，蛋白质构象会议上，我们将给出 研讨会的广度比其前辈更大，包括 除结晶和衍射技术外的技术 方法-已成功用于获取 关于膜蛋白结构的信息。四天的他 研讨会将侧重于技术集群：(1)洗涤剂， 膜蛋白性质及纯化，(2)膜 蛋白质结晶和衍射法(3) 膜蛋白的光谱分析，(4)实验 膜蛋白研究的预测方法 二级结构。这四天中的每一天都有下午 实验室和/或演示是对该主题的补充 上午讲座的事。最后一天早上 会议将致力于深入审查几个 膜蛋白已经被各种不同的 结构方法。

The central goal of the project proposed here is the acceleration of progress in structural studies of membrane proteins through the dissemination of information on a variety of biophysical techniques that have been successfully applied to membrane protein studies. The specific method that we propose for the dissemination of information is a workshop which we propose to hold October 13-18, 1990, on the campus of the Vanderbilt University School of Medicine. This workshop follows the two "American Schools on the Crystallization of Membrane Proteins" held in December 1985, in Boulder, Colorado, and in October 1987, at the Argonne National Laboratory, and the conference on "Experimental and Theoretical Aspects of the Interactions the Determine Protein Conformation" held in January 1989, at the National Institution of Health. LIke the first and second American Schools on the Crystallization of Membrane Proteins, we have chosen a workshop format, so that participants can receive hand-on experience with the methods under discussion. Like the conference on Protein Conformation, however, we will give the workshop greater breadth than its predecessors by including techniques-in addition to crystallization and diffraction methods-that have been successfully used for obtaining information about membrane protein structure. Four days of he workshop will focus on clusters of techniques: (1) detergents, membrane protein properties and purification, (2) membrane protein crystallization and diffraction methods, (3) spectroscopic analyses of membrane proteins, and (4) experimental and predictive methods for investigating membrane protein secondary structure. Each of those four days will have afternoon laboratories and/or demonstrations that compliment the subject matter of the morning lecture. The final morning of the conference will be devoted to in-depth reviews of several membrane proteins which have been studied by a variety of structural methods.