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POPULATION GENETICS OF XANTHINE DEHYDROGENASE EXPRESSION

黄嘌呤脱氢酶表达的群体遗传学

基本信息

批准号：
3438813
负责人：
KAZUO HIRAIZUMI
金额：
$ 12.23万
依托单位：
GETTYSBURG COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-05-01 至 1993-04-30
项目状态：
已结题

项目摘要

An active area of research in population genetics concerns the hypothesis that adaptive evolutionary changes reflect modifications in the level or timing of the expression of structural genes more than changes in the structural genes themselves or their translational products. This hypothesis suggests that variation of genetic elements involved in gene regulation would play a more significant role in evolution and adaptation than structural gene variation. This proposal will initiate a natural population genetics study on gene regulation to evaluate this hypothesis, using as a model the rosy-xanthine dehydrogenase (XDH) system in Drosophila melanogaster. The adaptive potential of XDH activity variation is indicated by the well-documented observations of files that are either deficient or low in XDH activity not surviving under certain environmental conditions. The project is designed to accomplish the following. First, the extent of phenotypic by performing fluorometric assays for XDH. Second, these strains will be subjected to rocket immunoelectrophoresis to quantitate differences in the number of XDH molecules. Third, these strains will be assayed for XDH structural variation by sequential polyacrylamide gele electrophoresis. Finally, since these lines already have been characterized extensively for restriction sites around the structural gene are being sequenced, the data from this proposed research will be used to study relationships among phenotypic variation, XDH protein variation, and DNA sequence variation in the control (regulatory) element as opposed to structural element of this enzyme-coding gene. The results will contribute toward the understanding of the relative phenotypic effects of mutations (caused by UV or high energy irradiation or chemical mutagens) in regulatory versus structural sequences in human populations.

群体遗传学的一个活跃的研究领域是关于适应性进化的变化反映了水平的变化，结构基因表达的时间比结构基因表达的时间结构基因本身或其翻译产物。这一种假说认为，参与基因的遗传元件的变异调控将在进化和适应中发挥更重要的作用结构基因变异。这项提议将启动一个自然的关于基因调控的群体遗传学研究为了评估这一假设，以果蝇中的玫瑰黄嘌呤脱氢酶（XDH）系统为模型，黑腹菌 XDH活性变化的适应潜力是由文件的记录良好的观察结果表明， XDH活性缺乏或低，在某些环境下不能存活条件该项目旨在实现以下目标。第一、通过对XDH进行荧光测定来确定表型的程度。其次，这些菌株将进行火箭免疫电泳，定量XDH分子数量的差异。第三，这些将通过连续测定菌株的XDH结构变异，聚丙烯酰胺凝胶电泳最后，由于这些线已经已经被广泛地表征为限制性位点周围的结构基因正在测序，这项研究的数据将用于研究表型变异、XDH蛋白质控制（调节）元件中的DNA序列变异而不是这种酶编码基因的结构元件。结果将有助于理解相对表型效应突变（由紫外线或高能辐射或化学诱变剂引起）调节序列和结构序列的差异。