FOURIER AND EIGENSHAPE ANALYSIS OF MOSQUITO EGG SHAPE

蚊卵形状的傅立叶和本征形分析

• 批准号: 2067619
• 负责人: LARRY G PAPPAS
• 金额: $ 6.52万
• 依托单位: PERU STATE COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1994-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2067619
• 关键词: Aedes; animal population study; egg /ovum; field study; image processing; light microscopy; mathematical model; morphology; species difference; statistics /biometry; systematic biology

DESCRIPTION (Adapted from the Applicant's Abstract): This application under the Academic Research Enhancement Award (AREA) program proposes research which would endeavor to develop modern methods for the differentiation of species and populations of mosquito which breed in treeholes and other containers both natural and man-made. The project stresses the development of new morphometric methods based on mathematical shape analysis for identifying the ova of the aedine species in question. An automated system for data acquisition and analysis is also proposed for development. This project has three long-term goals. The first of these is to develop new, unique morphometric characters associated with the egg stage which can be used to identify species of Aedes which utilize treeholes and containers for oviposition and larval development. In order to test the hypothesis that species can be delineated using egg shape the investigators will first endeavor to develop an automated system for routine collection and analysis of data on egg size and shape and chorionic sculpturing. Technical aspects of this task will be accomplished by employing a charge coupled device (CCD) to send microscopic images of eggs to a frame grabber, digitizing tablet and computer. Fourier and eigenshape analysis will be conducted using specialized software, and statistical comparisons of the resulting parameters between groups will be made. To be scrutinized by these methods are Aedes triseriatus, Ae. hendersoni, and Ae. albopictus, species presently maintained in the applicant's laboratory, and Ae. atropalpus, Ae. aegypti and Ae. bahamensis for which starting material will be provided by collaborators. Eggs for analysis will be obtained from individual bloodfed females. In addition to interspecific studies, the aforementioned methods will be applied to the separation of geographic populations within species based on egg size and shape. These studies will test the hypotheses that geographic variation can be demonstrated in egg shape parameters and that the relatedness of populations can be deduced from these data. Various geographic strains of Aedes triseriatus, Ae. hendersoni and Ae. albopictus are available in the applicant's laboratory for these studies. The second long term goal of the project is to provide the basis for further research beyond this project. This work will utilize the automated system to investigate chorionic sculpturing patterns as possible bases for species and population identification. The relationship of egg shape and size to geographic location, environmental conditions and seasonality will be studied. Finally, the effect of hybridization on egg shape variability will be investigated. The final large objective of the proposal is to train undergraduate students in mathematically and statistically based morphological systematics. The progress report lists numerous students from the applicant's program who have successfully completed research projects and who have won student research competitions sponsored by national scientific societies.

描述（改编自申请人的摘要）： 根据学术研究促进奖（AREA）计划， 研究将努力发展现代方法， 蚊子的种类和种群的分化， 树洞和其他自然和人造的容器。 项目 强调新的形态测量方法的发展， 形状分析用于鉴定所讨论的伊蚊属物种的卵。 还提出了一种用于数据采集和分析的自动化系统， 发展 该项目有三个长期目标。 第一个是发展 与卵期相关的新的、独特的形态特征， 可用于识别利用树洞的伊蚊种类， 用于产卵和幼虫发育的容器。 为了测试 假设物种可以用蛋形来描绘， 研究人员将首先努力开发一个自动化系统， 常规收集和分析鸡蛋大小和形状的数据， 绒毛膜雕刻 这项任务的技术方面将是 通过采用电荷耦合器件（CCD）来发送 鸡蛋的显微图像到一个图像采集器，数字化平板电脑， 电脑 傅立叶和特征形状分析将使用 专门的软件，并对结果进行统计比较 组之间的参数。 被这些 方法是三列伊蚊（Aedes tristeriatus）、Ae. hendersoni和山慈菇Ae.白纹伊蚊属 目前保存在申请人的实验室，和Ae。无须的， AE.埃及伊蚊和埃及伊蚊。巴哈马，其起始材料将 由合作者提供。 用于分析的鸡蛋将从 单独的吸血雌性 除了种间研究， 上述方法将应用于地理分离 根据卵的大小和形状来划分种群。 这些研究 将测试的假设，地理变异可以证明， 蛋形参数和种群的相关性可以 从这些数据中推断出来。 三列伊蚊的不同地理株， AE. hendersoni和Ae.白纹伊蚊可在申请人的 这些研究的实验室。 该项目的第二个长期目标是提供基础， 进一步研究这个项目。 这项工作将利用 自动化系统，以研究绒毛膜雕刻模式， 物种和种群识别的基础。 鸡蛋的关系 形状和大小的地理位置，环境条件和 将研究季节性。 最后，杂交对卵子的影响 将研究形状可变性。 该提案的最后一个大目标是培养本科生 学生在数学和统计为基础的形态学 分类学 进度报告列出了许多学生从 成功完成研究项目的申请人计划， 他们赢得了由国家赞助的学生研究比赛， 科学社会。