MOLECULAR AND SOMATIC CELL MAPPING OF DEER MOUSE GENES

重组DNA技术的最新进展（包括 聚合酶链反应方案）和体细胞中 遗传学，再加上经典的生物化学遗传学， 细胞遗传学，提供了一个前所未有的机会，扩大我们的 哺乳动物基因组组织的知识。 鹿鼠 （属Peromyscus）被认为是理想的模式， 比较测绘工作有几个原因。 比如鹿 小鼠的细胞遗传学特征非常好， 在很大程度上负责核型进化的机制， 灵长类动物和鹿鼠（臂间倒位和 异色添加）显然是相同的。 因此，任何 所获得的信息将与人类直接相关。 与 实验室小鼠和大鼠除外，临床上 对鹿鼠的研究比其他任何动物都多 模型 鹿鼠基因图谱的扩展将与 并将促进新的研究倡议，在广泛的 重要的生物医学研究领域。 此外，发展 鹿鼠图的基本原理 生物学问题1)重组抑制是否 臂间倒位是一种保护基因的机制 在这些区域内，？2)是染色体显带 物种之间的相似性指示潜在的基因 相似性;和3）哺乳动物基因组的部分被 是偶然的还是选择的

Recent advances in recombinant DNA technology (including polymerase chain reaction protocols) and in somatic cell genetics, coupled with classic biochemical genetics and cytogenetics, offer an unprecedented opportunity to expand our knowledge of mammalian genome organization. The deer mouse (genus Peromyscus) is considered the ideal model for directed comparative mapping efforts for several reasons. For one, deer mice are extremely well characterized cytogenetically, and the mechanisms largely responsible for karyotypic evolution in primates and deer mice (pericentric inversions and heterochromatic additions) are apparently identical. Thus, any information obtained will have direct human relevance. With the exceptions of the laboratory mouse and rat, more clinical research is performed on the deer mouse than any other animal model. Expansion of the deer mouse gene map will have relevance and will facilitate new research initiatives in a wide range of biomedically important research areas. In addition, development of a deer mouse map will address the following fundamental biological questions.. 1) Does recombination inhibition within pericentric inversions function as a mechanism to protect gene combinations within these regions? 2) Is chromosome banding similarity among species indicative of underlying genic similarity; and 3) Have portions of the mammalian genome been conserved by chance or by selection?