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CONTROL OF CAPILLARY ENDOTHELIAL CELL ANTIGEN EXPRESSION

毛细血管内皮细胞抗原表达的控制

基本信息

批准号：
3440005
负责人：
DALE E GREENWALT
金额：
$ 6.88万
依托单位：
SAN JOSE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-05-01 至 1990-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3440005
关键词：
antibody antibody specificity cell cell interaction gel electrophoresis gene expression human tissue immunofluorescence technique immunological substance neoplastic transformation surface antigens vascular endothelium

项目摘要

The long-term objective is to elucidate the mechanisms which give rise to endothelial cell heterogeneity. Different endothelia (e.g. myocordial capillary endothelia vs. lung capillary endothelia) have been shown to possess different cell surface antigens. An hypothesis has been proposed which states that the endothelia of new vascular ingrowth becomes specialized in response to the unique characteristics of the surrounding epithelium. This proposal is designed to allow preliminary examination of that hypothesis and form the basis for subsequent more detailed investigations. Advantage will be taken of the presence of a capillary endothelial cell surface glycoprotein (PAS IV) which, in bovine tissues, is present in some epithelial tissues (eg. heart, liver, and mammary gland) but not others (eg. lung and brain). A similar if not identical glycoprotein is present in human tissues. PAS IV will be purified from human tissue and antibodies specific for human PAS IV will be produced and characterized. Evidence will be sought for host tissue control of PAS IV expression through immunofluorescent localization and antigen characterization studies. Endothelial cell surface heterogeneity is undoubtedly a product of tissue - specific endothelial cell function. Knowledge of the basis for the surface antigen heterogeneity will aid our understanding of endothelial cell function. These studies are of general biological significance and may assist in our understanding of cell-cell and cell-matrix interactions between other cell types.

长期目标是阐明给予的机制导致内皮细胞异质性。不同的内皮细胞（例如心肌毛细血管内皮与肺毛细血管内皮）有已被证明具有不同的细胞表面抗原。一个已提出假设，指出内皮细胞新的血管向内生长变得专门化以响应独特的周围上皮的特征。这个提议是旨在允许对该假设进行初步检验，并且为后续更详细的调查奠定基础。将利用毛细血管内皮细胞的存在细胞表面糖蛋白（PAS IV），在牛组织中，存在于一些上皮组织（例如心脏、肝脏和乳腺）腺）但不是其他（例如肺和脑）。如果没有类似的人体组织中存在相同的糖蛋白。 PAS IV 将从人类组织和人类 PAS 特异性抗体中纯化 IV 将被生产并表征。将寻求证据通过免疫荧光宿主组织控制 PAS IV 表达定位和抗原表征研究。内皮细胞表面异质性无疑是以下因素的产物组织-特异性内皮细胞功能。的知识表面抗原异质性的基础将有助于我们了解内皮细胞功能。这些研究是一般生物学意义，可能有助于我们的理解其他细胞类型之间的细胞-细胞和细胞-基质相互作用。