ONCOGENESIS OF BLADDER EPITHELIAL CELLS

膀胱上皮细胞的癌发生

• 批准号: 3446957
• 负责人: IAN CHARLES SUMMERHAYES
• 金额: $ 6.11万
• 依托单位: NEW ENGLAND DEACONESS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446957
• 关键词: athymic mouse; bladder neoplasm; cancer registry /resource; extracellular matrix; gene expression; genetic manipulation; human tissue; laboratory mouse; molecular cloning; monoclonal antibody; neoplasm /cancer genetics; neoplastic transformation; nucleic acid sequence; oncogenes; oncoproteins; protein biosynthesis; scanning electron microscopy; tissue /cell culture; transfection; urinary bladder epithelium

The aims of this project are to investigate the natural history of bladder cancer in mice and man using cell and molecular biological approaches. The central approach to this work is the use of gene transfer techniques to study the effects of known transforming genes in different bladder epithelial cell lines, both mouse and human, and to look for alternative routes of transformation in bladder cancer. The aims of the proposal are: 1. To identify alternative routes of transformation in bladder cancer using NIH/3T3 cells with in vivo selection and by developing new recipients for DNA transfection. 2. To study the expression of characterized transforming genes in different epithelial cell types to establish the effects of the differentiation and growth characteristics of these cells in vitro and in vivo. 3. To examine the timing and expression of ras gene products in bladder carcinogenesis and to study the mode of action of different transforming genes and define cellular targets involved in epithelial transformation.

本项目的目的是调查膀胱的自然史 用细胞和分子生物学方法研究小鼠和人的癌症。 的 这项工作的核心方法是使用基因转移技术， 研究已知转化基因在不同膀胱中的作用 上皮细胞系，小鼠和人类，并寻找替代 膀胱癌的转化途径。 该提案的目的是： 1.为了确定膀胱癌转化的替代途径， NIH/3 T3细胞与体内选择和发展新的受体， DNA转染。 2.研究不同转化基因在不同细胞中的表达情况， 上皮细胞类型，以确定分化的影响， 这些细胞在体外和体内的生长特性。 3.探讨ras基因产物在膀胱中表达的时间和表达 研究不同转化途径的作用方式 基因和定义参与上皮转化的细胞靶点。