HEPARAN SULFATE EFFECTS ON ENDOTHELIAL CELL BEHAVIOR

内皮细胞位于血液的管腔表面 血管，并参与许多重要的功能和一些 病态的国家 内皮细胞层的糖萼 提供低血栓形成性质的表面。 一个重要 这一层的组成部分是高度负电荷 内皮硫酸乙酰肝素。 内源性硫酸乙酰肝素和 肝素在体外通过 一种能识别两种分子的受体。 内皮肝素 硫酸盐和肝素抑制血管平滑肌细胞 扩散和迁移。 此外，过度矩阵 在动脉粥样硬化中发生的积聚是值得注意的， %的硫酸乙酰肝素。 肝素的内皮受体 硫酸盐可能与细胞外基质硫酸乙酰肝素相互作用 除了糖萼硫酸乙酰肝素之外的蛋白聚糖 蛋白聚糖 这项研究计划旨在研究细胞 对配体的存在或不存在作出反应的活性 （硫酸乙酰肝素蛋白聚糖或肝素）对硫酸乙酰肝素 受体。 这些研究将有助于更好地了解 内皮细胞控制的硫酸乙酰肝素浓度 糖萼和内膜基质。

Endothelial cells are located at the luminal surface of blood vessels and are involved in many important functions and some diseased states. The glycocalyx of the endothelial cell layer provides a surface of low thrombogenic nature. One important component of this layer is the highly negatively charged endothelial heparan sulfate. Both endogenous heparan sulfate and heparin are bound to endothelial cell surfaces in vitro through a receptor which recognizes both molecules. Endothelial heparan sulfate and heparin inhibit vascular smooth muscle cell proliferation and migration. In addition, the excessive matrix build-up that occurs in atherosclerosis is notable for its decreased percent of heparan sulfate. The endothelial receptors for heparan sulfate may interact with extracellular matrix heparan sulfate proteoglycans in addition to the glycocalyx heparan sulfate proteoglycans. This research proposal aims to study the cell activities which respond to the absence or presence of ligands (heparan sulfate proteoglycans or heparin) on the heparan sulfate receptors. These studies will lead to a better understanding of endothelial cell control of heparan sulfate concentrations in the glycocalyx and intimal matrix.