ONCOGENESIS OF BLADDER EPITHELIAL CELLS

膀胱上皮细胞的癌发生

• 批准号: 3446956
• 负责人: IAN CHARLES SUMMERHAYES
• 金额: $ 6.04万
• 依托单位: NEW ENGLAND DEACONESS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446956
• 关键词: bladder neoplasm; cancer registry /resource; extracellular matrix; gene expression; genetic manipulation; human tissue; molecular cloning; monoclonal antibody; neoplasm /cancer genetics; neoplastic transformation; nucleic acid sequence; oncogenes; oncoproteins; scanning electron microscopy; tissue /cell culture; urinary bladder epithelium

The aims of this project are to investigate the natural history of bladder cancer in mice and man using cell and molecular biological approaches. The central approach to this work is the use of gene transfer techniques to study the effects of known transforming genes in different bladder epithelial cell lines, both mouse and human, and to look for alternative routes of transformation in bladder cancer. The aims of the proposal are: 1. To identify alternative routes of transformation in bladder cancer using NIH/3T3 cells with in vivo selection and by developing new recipients for DNA transfection. 2. To study the expression of characterized transforming genes in different epithelial cell types to establish the effects of the differentiation and growth characteristics of these cells in vitro and in vivo. 3. To examine the timing and expression of ras gene products in bladder carcinogenesis and to study the mode of action of different transforming genes and define cellular targets involved in epithelial transformation.

这个项目的目的是研究膀胱的自然历史