STUDY OF FLUID PINOCYTOSIS IN STIMULATED NEUTROPHILS

液体胞饮是最近描述的中性粒细胞的一种细胞事件， 急性炎症的主要细胞成分，作为对 用趋化因子(CF)刺激。发生胞饮增多症 与Fc诱导的其他中性粒细胞事件同时发生 运动性的或运动性的反应，与早期事件相反，如 对CF的氧化代谢和分泌反应突然爆发。它是 通过我们以前的工作推测，CF诱导的胞饮作用是 与运动的细胞事件有关，而不仅仅是与CF有关 配体-受体复合体内化和受体循环。本研究 是为了进一步描述CF诱导的胞饮作用的过程 中性粒细胞；调查与以下事件有关的现象 Cf的运动和膜受体的调节；并研究 髓系细胞、髓系白血病细胞的发育过程 已知中性粒细胞功能障碍的临床状态的中性粒细胞。 液体吞噬作用将使用荧光流式细胞仪进行测量 测定和放射性标记的液体相标志物摄取试验。超微结构 形态和生化亚细胞分级方法将是 使用酶标记、放射性标记和形态标记 胞饮作用的药理抑制剂研究亚细胞 这一过程的过程及其与吸附内吞作用的关系。这个 膜扰动对膜流动性的影响 用荧光偏振度测量，及其与流体的关系 将对胞饮作用进行研究。用荧光探针和 用于检查中性粒细胞中液体吞噬作用的药物 趋化性将被用来研究两者之间的相互关系。 流程。发育中髓系吞饮细胞增多的流式细胞术分析 液体细胞诱导分化的细胞和白血病细胞系 培养将被用来描述未成熟髓系的过程。 细胞。希望这些研究将描述和定义 中性粒细胞刺激性液体吞噬的生物学意义。(MB)

Fluid pinocytosis is a recently described cellular event of neutrophils, the major cellular component of acute inflammation, in response to stimulation with chemotactic factors (CF). Pinocytosis occurs simultaneously with other FC-induced neutrophil events relating to the kinetic or locomotory response in contrast to early events, such as the burst of oxidative metabolism and secretory response to CF. It is postulated through our previous work that the CF-induced pinocytosis is related to the cellular events of locomotion and not solely to CF ligand-receptor complex internalization and receptor recycling. This study is to further characterize the process of CF-induced pinocytosis neutrophils; investigate the phenomenon with regard to the events of locomotion and the modulation of membrane receptors for CF; and study the process in developing myeloid cells, myeloid leukemia cells, and neutrophils from clinical states of known neutrophil dysfunction. Fluid pinocytosis will be measured using a fluorescent flow cytometric assay and radiolabeled, fluid phase marker uptake assays. Ultrastructural morphologic and biochemical subcellular fractionation methods will be employed using enzymatic, radioactive, and morphologic markers and pharmacologic inhibitors of pinocytosis to study the subcellular processing and relationship to adsorptive endocytosis of this process. The effects of membrane perturbations in regard to membrane fluidity, as measured by fluorescence polarization, and its relationship to fluid pinocytosis will be studied. Studies with fluorescent probes and pharmacologic agents to examine fluid pinocytosis during neutrophil chemotaxis will be employed to investigate the interrelationship of the two processes. Flow cytometric analysis of pinocytosis in developing myeloid cells and leukemic cell lines induced to differentiate in liquid cell culture will be used to characterize the process in immature myeloid cells. lt is hoped that these studies will characterize and define the biologic significance of stimulated fluid pinocytosis in neutrophils. (MB)