ALCOHOL & PANCREATIC DIGESTIVE ENZYME SYNTHESIS

拟议的研究计划的长期目标是获得 更好地了解在什么情况下 长期饮酒改变胰腺的合成 消化酶。个体消化合成的速率 将对对照组和酒精喂养的大鼠的酶进行比较 体内和体外，在分离的腺泡细胞制剂中。利率 单个消化酶的合成将由以下因素决定 研究标记氨基酸在蛋白质中的掺入。 单个蛋白质的分离将通过以下方式实现 聚丙烯酰胺凝胶电泳法。乙醇对人体的影响 摄食对消化酶周转的影响 持续暴露在乙醇中，而乙醇在其中 隔夜取款也将接受调查。这种可能性 酒精摄入对胰腺蛋白质的影响 合成和周转是由释放的 胃肠激素如缩胆囊素和促胰液素会 接受测试。假设在长期摄入乙醇的过程中， 这种促分泌剂的作用被胰腺所补偿。 胰多肽和生长抑素等抑制物将 也要接受测试。由于胰腺消化的成分 已知的酶主要受组成成分的调节 在饮食中，适当的饮食控制将被包括在 区分饮酒的具体影响和那些 其他饮食因素的影响。预计总的结果是 建议的研究将提供重要的新信息 关于胰腺对糖尿病发展的易感性 酒精摄入对大鼠胰腺炎的影响 消化酶合成。

The long-term goals of the proposed research plan are to gain a better understanding of the biochemical circumstances under which chronic alcohol consumption modifies the synthesis of pancreatic digestive enzymes. The rate of synthesis of individual digestive enzymes will be compared between control and ethanol-fed rats in vivo and in vitro, in isolated acinar cell preparations. The rate of synthesis of individual digestive enzymes will be determined by studying the incorporation of labelled amino acids into proteins. Separation of individual proteins will be achieved by polyacrylamide gel-electrophoresis. The effects of ethanol ingestion on the turnover of digestive enzymes in animals that are continuously exposed to ethanol and those in which ethanol is withdrawn overnight will also be investigated. The possibility that the effects of ethanol ingestion on pancreatic protein synthesis and turnover is mediated by the release of gastrointestinal hormones such as cholecystokinin and secretin will be tested. The hypothesis that during long-term ethanol intake, the effects of such secretagogues are compensated by pancreatic inhibitors such as pancreatic polypeptide and somatostatin will also be tested. Since the composition of pancreatic digestive enzymes is known to be predominantly regulated by the composition of the diet, appropriate dietary controls will be included to distinguish specific effects of alcohol administration from those of other dietary factors. It is expected that the overall outcome of the proposed studies will provide significant new information on the susceptibilities of the pancreas to the development of pancreatitis mediated by the effects of ethanol ingestion on digestive enzyme synthesis.