ALCOHOL & PANCREATIC DIGESTIVE ENZYME SYNTHESIS

拟议研究计划的长期目标是获得 更好地了解生物化学环境， 慢性酒精消耗改变胰腺的合成 消化酶 个体消化道蛋白质的合成速率 酶将在对照组和乙醇喂养的大鼠之间进行比较， 在分离的腺泡细胞制备物中，在体内和体外。 率 单个消化酶的合成将由 研究标记氨基酸与蛋白质的结合。 单个蛋白质的分离将通过 聚丙烯酰胺凝胶电泳 乙醇的影响 摄食对动物消化酶周转的影响， 持续暴露于乙醇和其中乙醇 连夜撤离的也将被调查。 的可能性 酒精摄入对胰腺蛋白质的影响 合成和周转是通过释放 胃肠激素如胆囊收缩素和促胰液素将 得到考验 假设在长期摄入乙醇的过程中， 这种促分泌素的作用由胰腺分泌素补偿， 抑制剂如胰多肽和生长抑素将 也被测试。 由于胰腺消化道的组成 已知酶主要受组合物调节 适当的饮食控制将包括在内， 区分酒精管理的具体影响， 其他饮食因素。 预计总体成果 将提供重要的新信息 关于胰腺对 酒精摄入对胰腺炎的影响 消化酶合成

The long-term goals of the proposed research plan are to gain a better understanding of the biochemical circumstances under which chronic alcohol consumption modifies the synthesis of pancreatic digestive enzymes. The rate of synthesis of individual digestive enzymes will be compared between control and ethanol-fed rats in vivo and in vitro, in isolated acinar cell preparations. The rate of synthesis of individual digestive enzymes will be determined by studying the incorporation of labelled amino acids into proteins. Separation of individual proteins will be achieved by polyacrylamide gel-electrophoresis. The effects of ethanol ingestion on the turnover of digestive enzymes in animals that are continuously exposed to ethanol and those in which ethanol is withdrawn overnight will also be investigated. The possibility that the effects of ethanol ingestion on pancreatic protein synthesis and turnover is mediated by the release of gastrointestinal hormones such as cholecystokinin and secretin will be tested. The hypothesis that during long-term ethanol intake, the effects of such secretagogues are compensated by pancreatic inhibitors such as pancreatic polypeptide and somatostatin will also be tested. Since the composition of pancreatic digestive enzymes is known to be predominantly regulated by the composition of the diet, appropriate dietary controls will be included to distinguish specific effects of alcohol administration from those of other dietary factors. It is expected that the overall outcome of the proposed studies will provide significant new information on the susceptibilities of the pancreas to the development of pancreatitis mediated by the effects of ethanol ingestion on digestive enzyme synthesis.