ONCOGENESIS OF BLADDER EPITHELIAL CELLS

膀胱上皮细胞的癌发生

• 批准号: 3446955
• 负责人: IAN CHARLES SUMMERHAYES
• 金额: $ 6.36万
• 依托单位: NEW ENGLAND DEACONESS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446955
• 关键词: bladder neoplasm; cancer registry /resource; extracellular matrix; gene expression; genetic manipulation; human tissue; molecular cloning; monoclonal antibody; neoplasm /cancer genetics; neoplastic transformation; nucleic acid sequence; oncogenes; oncoproteins; scanning electron microscopy; tissue /cell culture; urinary bladder epithelium

The aims of this project are to investigate the natural history of bladder cancer in mice and man using cell and molecular biological approaches. The central approach to this work is the use of gene transfer techniques to study the effects of known transforming genes in different bladder epithelial cell lines, both mouse and human, and to look for alternative routes of transformation in bladder cancer. The aims of the proposal are: 1. To identify alternative routes of transformation in bladder cancer using NIH/3T3 cells with in vivo selection and by developing new recipients for DNA transfection. 2. To study the expression of characterized transforming genes in different epithelial cell types to establish the effects of the differentiation and growth characteristics of these cells in vitro and in vivo. 3. To examine the timing and expression of ras gene products in bladder carcinogenesis and to study the mode of action of different transforming genes and define cellular targets involved in epithelial transformation.

该项目的目的是研究膀胱的自然史 使用细胞和分子生物学方法对小鼠和人类进行癌症研究。 这 这项工作的核心方法是利用基因转移技术 研究已知转化基因在不同膀胱中的影响 上皮细胞系，包括小鼠和人类，并寻找替代品 膀胱癌的转化途径。 该提案的目的是： 1. 确定膀胱癌的替代转化途径 NIH/3T3 细胞通过体内选择并开发新的受体 DNA转染。 2. 研究特征转化基因在不同物种中的表达情况 上皮细胞类型以确定分化和 这些细胞在体外和体内的生长特征。 3. 检查膀胱中ras基因产物的时间和表达 致癌作用并研究不同转化的作用方式 基因并定义参与上皮转化的细胞靶标。