NEURAL REGULATION OF THE ENDOCRINE PANCREAS

我们的目标是更好地理解这些机制 导致老年人血糖动态平衡恶化。我们建议 承接了一套集形态、功能、生化为一体的 老年人内分泌胰腺及其自主神经支配的研究 分别于3月龄、6月龄、12月龄、18月龄、24月龄和30月龄C57BL/6NNia小鼠： 定义与年龄相关的结构和功能的变化 该动物模型中的胰岛，以及2)确定 年龄相关的胰岛结构和功能改变是由于 改变了胰腺的自主神经输入。 将对胰岛的一般形态进行评估。 定性地使用常规的光学显微镜技术 (乙醛-品红-三色)。胰岛A、B、D和PP细胞将 通过免疫细胞化学进行鉴定。胆碱能和肾上腺素能神经 胰腺中的纤维将使用乙酰胆碱酯酶和 乙醛酸-荧光组织化学。音量 胰岛密度和胰岛内神经纤维线密度 胰腺将使用体视学技术进行检测 (计算机辅助形态测量学)。血浆胰岛素和胰升糖素对 将确定禁食、禁食后再进食和血糖负荷 使用放射免疫分析。胆碱能和肾上腺素能活性 胰腺内的神经将通过测量，在提取的 胰腺组织，生物合成酶的活性 胆碱乙酰转移酶(CAT)和酪氨酸羟基酶(TOH)， 分别进行了分析。数据将在不同年龄组之间进行比较，使用以下分析 方差之后进行Newman-Kuels检验以确定年龄对 每个参数都进行了测量。

Our objective is to provide a better understanding of the mechanisms leading to deterioration of glucose homeostasis in the elderly. We propose to undertake a set of integrated morphological, functional and biochemical studies of the endocrine pancreas and its autonomic innervation in the C57BL/6NNia mouse at 3, 6, 12, 18, 24 and 30 months of age in order to: 1) define age-correlated changes in the structure and function of the pancreatic islets in this animal model, and 2) determine whether age-correlated changes in pancreatic islet structure and function are due to altered autonomic input to the pancreas. The general morphology of the pancreatic islets will be assessed qualitatively using routine light microscopic techniques (aldehyde-fuchsin-trichrome). Pancreatic islet A, B, D and PP cells will be identified using immunocytochemistry. Cholinergic and adrenergic nerve fibers in the pancreas will be identified using acetylcholinesterase and glyoxylic acid-fluorescence histochemistry, respectively. The volume density of islets and the linear density of nerve fibers within the pancreas will be detemrined using stereological techniques (computer-assisted morphometry). Plasms insulin and glucagon responses to fasting, refeeding after a fast, and to glucose loads will be determined using radioimmunoassays. The activities of cholinergic and adrenergic nerves within the pancreas will be determined by measuring, in extracts of pancreatic tissues, the activities the biosynthetic enzymes cholineacetyl-transferase (CAT) and tyrosine hydroxylase (TOH), respectively. Data will be compared between age groups, using analysis of variance followed by Newman-Kuels tests to determine the effect of age on each parameter measured.