NEURAL REGULATION OF THE ENDOCRINE PANCREAS

我们的目标是更好地了解这些机制， 导致老年人葡萄糖稳态的恶化。 我们提出 进行一套综合的形态、功能和生化 大鼠内分泌胰腺及其自主神经支配的研究 在3、6、12、18、24和30月龄的C57 BL/6 NNia小鼠中，以：1） 定义年龄相关的结构和功能的变化， 在该动物模型中的胰岛，和2）确定是否 胰岛结构和功能的年龄相关性变化是由于 改变了对胰腺的自主输入 将评估胰岛的一般形态学 使用常规光学显微镜技术定性 （三色品红）。 胰岛A、B、D和PP细胞将 使用免疫细胞化学进行鉴定。 胆碱能和肾上腺素能神经 胰腺中的纤维将使用乙酰胆碱酯酶进行鉴定， 乙醛酸荧光组织化学法。 体积 胰岛的密度和神经纤维的线密度 将使用体视学技术确定胰腺 （计算机辅助形态测定）。 血浆胰岛素和胰高血糖素对 空腹、禁食后再进食和葡萄糖负荷将被确定 使用放射免疫分析。 胆碱能和肾上腺素能的活性 胰腺内的神经将通过测量 胰腺组织，生物合成酶的活性 胆碱乙酰转移酶（CAT）和酪氨酸羟化酶（TOH）， 分别 数据将在年龄组之间进行比较，使用以下分析 方差分析，然后进行Newman-Kuels检验，以确定年龄对 测量的每一个参数。

Our objective is to provide a better understanding of the mechanisms leading to deterioration of glucose homeostasis in the elderly. We propose to undertake a set of integrated morphological, functional and biochemical studies of the endocrine pancreas and its autonomic innervation in the C57BL/6NNia mouse at 3, 6, 12, 18, 24 and 30 months of age in order to: 1) define age-correlated changes in the structure and function of the pancreatic islets in this animal model, and 2) determine whether age-correlated changes in pancreatic islet structure and function are due to altered autonomic input to the pancreas. The general morphology of the pancreatic islets will be assessed qualitatively using routine light microscopic techniques (aldehyde-fuchsin-trichrome). Pancreatic islet A, B, D and PP cells will be identified using immunocytochemistry. Cholinergic and adrenergic nerve fibers in the pancreas will be identified using acetylcholinesterase and glyoxylic acid-fluorescence histochemistry, respectively. The volume density of islets and the linear density of nerve fibers within the pancreas will be detemrined using stereological techniques (computer-assisted morphometry). Plasms insulin and glucagon responses to fasting, refeeding after a fast, and to glucose loads will be determined using radioimmunoassays. The activities of cholinergic and adrenergic nerves within the pancreas will be determined by measuring, in extracts of pancreatic tissues, the activities the biosynthetic enzymes cholineacetyl-transferase (CAT) and tyrosine hydroxylase (TOH), respectively. Data will be compared between age groups, using analysis of variance followed by Newman-Kuels tests to determine the effect of age on each parameter measured.