Lab on a Stick: Smartphone-ready low cost rapid live microbe detection and identification using nanocoated Micro Capillary Film

Lab on a Stick：使用纳米涂层微毛细管薄膜进行智能手机就绪的低成本快速活体微生物检测和识别

• 批准号: EP/L013983/1
• 负责人: Alexander Edwards
• 金额: $ 12.55万
• 依托单位: University of Reading
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FL013983%2F1
• 关键词: Lab; Stick; Smartphone; ready; low

Overall project aim: simple, low cost detection of bacteria using an affordable new microfluidic technology Bacteria are a major subtype of microbes (or germs) that cause a range of infections and can also contaminate food and medicines during manufacturing. Tools for detecting bacteria are thus vital, but current methods are often laborious and slow, and require the use of a fully-equipped microbiology laboratory. This project will develop a new bacteria testing technology- called Lab-on-a-Stick - that makes detecting and identifying live bacteria simpler, faster and more portable at an affordable price. Ultimately Lab-on-a-Stick tests can be performed without lab equipment and read using a mobile phone camera.Technology background: Microfluidics and Lab-on-a-ChipResearch over recent decades has developed technology called "microfluidics" using tubes with dimensions smaller than 1mm, typically around 0.1-0.2 mm wide to process reagents and samples. One major aim of microfluidic research is to develop miniature tests, with advantages of the small size including faster, automatic tests and smaller samples. These are referred to as "Lab-on-a-Chip" because complex lab procedures can be performed on a tiny microchip device. However, Lab on a Chip products are expensive and difficult to manufacture and have so far been limited to specialised high value applications, and most rapid disposable tests still use a more familiar format- the dipstick- e.g. pregnancy or blood glucose tests. The great advantage of dipsticks is their extreme simplicity- just dip in sample, and wait for a colour change. Dipsticks have disadvantages, so ideally the benefits of Lab-on-a-Chip microfluidic technology should now be adapted into a dipstick form. New technology: Lab-on-a-StickA collaboration between Reading and Loughborogh Universities led to the invention an entirely new approach -called Lab-on-a-Stick - that promises to deliver microfluidic properties with the simplicity and low cost of a dipstick. This technology exploits special properties of a novel microfluidic material previously studied by the applicant, but also adds a new modification that applies the science of nanotechnology - in other words a modification with dimensions around 1/10,000th of a millimeter. The resulting nano-modified microfluidic material has many beneficial properties, including the ability to perform 10 different tests after dipping into one sample, and as it is exceptionally transparent the tests results can be measured using a mobile phone camera. Remarkably it can still be produced at very low cost, using a simple, conventional manufacturing process, allowing cheap disposable tests that deliver the power of microfluidics.Current project objectives:Unpublished studies have already proven that the new dipstick microfluidic technology can perform three standard blood tests in a single drop of blood, and shown that bacteria testing is also feasible. This grant will build on these preliminary studies to produce fully functioning devices for detecting harmful and infectious bacteria in a smartphone-friendly dipstick format. The study will focus on developing a fundamental understanding of how to use the nanotechnology modification to perform accurate and sensitive bacteria tests using the new Lab-on-a-Stick technology platform.

总体项目目标：使用负担得起的新型微流控技术对细菌进行简单、低成本的检测细菌是微生物(或细菌)的一个主要亚型，可引起一系列感染，也可在制造过程中污染食品和药品。因此，检测细菌的工具至关重要，但目前的方法往往费力且缓慢，需要使用设备齐全的微生物学实验室。该项目将开发一种新的细菌测试技术--称为棒上实验室--使检测和识别活细菌变得更简单、更快、更便携，而且价格合理。技术背景：微流体和芯片实验室近几十年来，研究人员利用尺寸小于1 mm的管子(通常为0.1-0.2 mm宽)来处理试剂和样品，开发出了一种称为“微流体”的技术。微流控研究的一个主要目标是开发微型测试，具有体积小的优点，包括快速、自动测试和较小的样品。这些被称为“芯片实验室”，因为复杂的实验室程序可以在一个微小的微芯片设备上执行。然而，芯片实验室产品价格昂贵，制造困难，到目前为止仅限于专门的高价值应用，而且大多数快速一次性测试仍然使用更熟悉的格式-试纸-例如怀孕或血糖测试。试纸的最大优点是它们极其简单--只需浸入样品，然后等待颜色变化。试纸有缺点，所以理想情况下，芯片实验室微流控技术的优点现在应该被改造成试纸形式。新技术：棒上实验室雷丁大学和拉夫堡大学的合作导致了一种全新的方法--被称为棒上实验室--承诺提供微流控特性，同时具有标尺的简单和低成本。这项技术利用了申请人先前研究的一种新型微流控材料的特殊性质，但也增加了一种应用纳米技术科学的新修改-换句话说，尺寸约为1/10,000毫米的修改。由此产生的纳米改性微流控材料具有许多有益的特性，包括在浸入一个样品后能够进行10种不同的测试，而且由于它非常透明，测试结果可以使用手机摄像头测量。值得注意的是，它仍然可以用非常低的成本生产，使用简单的传统制造工艺，允许廉价的一次性测试，提供微流体的力量。目前的项目目标：未发表的研究已经证明，新的试纸微流控技术可以在一滴血中进行三次标准血液测试，并表明细菌测试也是可行的。这笔赠款将建立在这些初步研究的基础上，以生产功能齐全的设备，以智能手机友好的试纸形式检测有害和传染性细菌。这项研究的重点将是发展对如何使用纳米技术修饰来进行准确和敏感的细菌测试的基本理解，使用新的棒上实验室技术平台。

项目成果

Label-free 1D microfluidic dipstick counting of microbial colonies and bacteriophage plaques.

对微生物菌落和噬菌斑进行无标记一维微流体试纸计数。

• DOI: 10.1039/d2lc00280a
• 发表时间: 2022
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Dönmez SI

Label-free smartphone quantitation of bacteria by darkfield imaging of light scattering in fluoropolymer micro capillary film allows portable detection of bacteriophage lysis

• DOI: 10.1016/j.snb.2020.128645
• 发表时间: 2020-11-15
• 期刊: SENSORS AND ACTUATORS B-CHEMICAL
• 影响因子: 8.4
• 作者: Donmez, Sultan Ilayda;Needs, Sarah H.;Edwards, Alexander D.
• 通讯作者: Edwards, Alexander D.

Rapid Bacterial Motility Monitoring Using Inexpensive 3D-Printed OpenFlexure Microscopy Allows Microfluidic Antibiotic Susceptibility Testing.

• DOI: 10.3390/mi13111974
• 发表时间: 2022-11-14
• 期刊: Micromachines
• 影响因子: 3.4
• 作者: Diep TT;Needs SH;Bizley S;Edwards AD
• 通讯作者: Edwards AD

Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays.

• DOI: 10.1021/acssensors.1c00704
• 发表时间: 2021-07-23
• 期刊: ACS sensors
• 影响因子: 8.9
• 作者: Barbosa AI;Edwards AD;Reis NM
• 通讯作者: Reis NM