TRANSFORMED T CELL LINES FOR STUDY OF T CELL MATURATION

用于研究 T 细胞成熟的转化 T 细胞系

• 批准号: 3446635
• 负责人: LAWRENCE K JUNG
• 金额: $ 5.31万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446635
• 关键词: B lymphocyte; T lymphocyte; antigens; bone marrow transplantation; cell cell interaction; cell differentiation; clone cells; enzyme linked immunosorbent assay; flow cytometry; helper T lymphocyte; histochemistry /cytochemistry; human T cell lymphotropic virus type 1; human subject; hybridomas; immunodeficiency; immunofluorescence technique; interleukin 2; leukocyte activation /transformation; mixed tissue /cell culture; monoclonal antibody; monokines; severe combined immunodeficiency; suppressor T lymphocyte; transforming virus; viral carcinogenesis

Although human T cell differentiation has been studied extensively using various markers such as differentiation antigens and lectin binding properties, careful functional analysis of these T cells at the various stages of differentiation has not been carried out. This is due to the difficulties in obtaining cells from one specific stage of differentiation in sufficient pure preparations. Although interleukin-2 (IL-2) can be used to clonally expand mature T cells, immature T cells are usually not responsive to such manipulation. As an alternative approach, human T cell leukemia virus (HTLV) infected cells have been used successfully to immortalize immature T cells from a patient with severe combined immunodeficiency, as well as normal mature T cells. This method thus offers the possibility that T cells of various stages of differentiation can be transformed, cloned and studied for their functional capacities and their expression of differentiation antigens. Hybridoma technology will be used to further characterize specific antigens of these stages of T cell differentiation. Functional analysis of such cells will include the study of their ability to proliferate in response to mitogens, interleukin-1 (IL-1) and IL-2. Their ability to produce lymphokinea will be studied with IL-2 microassay, B cell proliferation assay and B cell differentiation assay. T cell differentiation and maturation in normal individuals as well as in patients with primary immunodeficiencies and bone marrow recipients will be studied. Information thus obtained will be useful in the understanding of the events leading to the maturation of T cells. It will also shed light on the defects of maturation seen in patients with immunodeficiencies. Such insights will also be of critical importance in the analysis of T cell engraftment in bone marrow recipients. (LB)

虽然人类T细胞分化已经被广泛研究， 各种标记物如分化抗原和凝集素结合 这些T细胞的性质，仔细的功能分析，在不同的 分化阶段尚未进行。 这是由于 从一个特定的分化阶段获得细胞的困难 足够的纯制剂。 虽然白细胞介素-2（IL-2）可以用于 为了克隆扩增成熟T细胞，未成熟T细胞通常不 对这种操纵的反应。 作为一种替代方法，人类T细胞 白血病病毒（HTLV）感染的细胞已成功地用于 永生化来自患有严重的联合免疫缺陷的患者的未成熟T细胞， 免疫缺陷，以及正常的成熟T细胞。 该方法因此 提供了一种可能性，即不同分化阶段的T细胞 可以转化、克隆和研究其功能能力， 分化抗原的表达。 混合动力技术将成为 用于进一步表征T细胞的这些阶段的特异性抗原 分化 这些细胞的功能分析将包括研究 它们对有丝分裂原，白细胞介素-1 （IL-1）和IL-2。 他们产生淋巴细胞的能力将用 IL-2微量测定、B细胞增殖测定和B细胞分化 比色法 正常人的T细胞分化和成熟 如原发性免疫缺陷患者和骨髓接受者 将被研究。由此获得的信息将有助于 了解导致T细胞成熟的事件。 它将 还揭示了在患有糖尿病的患者中观察到的成熟缺陷， 免疫缺陷 这些见解也将是至关重要的， 骨髓受体T细胞植入分析。 （磅）