TRANSFORMED T CELL LINES FOR STUDY OF T CELL MATURATION

尽管人类T细胞分化已被广泛研究 分化抗原、凝集素结合等多种标记物 属性，仔细分析这些T细胞在不同时期的功能 分化阶段尚未进行。这是由于 从一个特定的分化阶段获取细胞的困难 在充足的纯制剂中。虽然可以使用白介素2(IL-2) 为了克隆扩增成熟的T细胞，未成熟的T细胞通常不会 对这种操纵做出反应的。作为一种替代方法，人类T细胞 白血病病毒(HTLV)感染细胞已成功用于 将一例重症联合白血病患者的未成熟T细胞永生化 免疫缺陷，以及正常的成熟T细胞。因此，这种方法 为不同分化阶段的T细胞提供了可能性 可以转化、克隆和研究它们的功能能力和 其分化抗原的表达。杂交瘤技术将是 用来进一步表征这些阶段的T细胞的特定抗原 差异化。对这些细胞的功能分析将包括这项研究 它们对有丝分裂原、白介素1的反应能力 (IL-1)和IL-2。他们产生淋巴运动的能力将被研究 IL-2微量检测、B细胞增殖实验和B细胞分化 化验。正常人T细胞的分化和成熟 与原发免疫缺陷患者和骨髓移植受者一样 将会被研究。这样获得的信息将对 了解导致T细胞成熟的事件。会的 也揭示了患者的成熟度缺陷。 免疫缺陷。这种洞察力也将在 T细胞在骨髓受者体内的植入情况分析(磅)

Although human T cell differentiation has been studied extensively using various markers such as differentiation antigens and lectin binding properties, careful functional analysis of these T cells at the various stages of differentiation has not been carried out. This is due to the difficulties in obtaining cells from one specific stage of differentiation in sufficient pure preparations. Although interleukin-2 (IL-2) can be used to clonally expand mature T cells, immature T cells are usually not responsive to such manipulation. As an alternative approach, human T cell leukemia virus (HTLV) infected cells have been used successfully to immortalize immature T cells from a patient with severe combined immunodeficiency, as well as normal mature T cells. This method thus offers the possibility that T cells of various stages of differentiation can be transformed, cloned and studied for their functional capacities and their expression of differentiation antigens. Hybridoma technology will be used to further characterize specific antigens of these stages of T cell differentiation. Functional analysis of such cells will include the study of their ability to proliferate in response to mitogens, interleukin-1 (IL-1) and IL-2. Their ability to produce lymphokinea will be studied with IL-2 microassay, B cell proliferation assay and B cell differentiation assay. T cell differentiation and maturation in normal individuals as well as in patients with primary immunodeficiencies and bone marrow recipients will be studied. Information thus obtained will be useful in the understanding of the events leading to the maturation of T cells. It will also shed light on the defects of maturation seen in patients with immunodeficiencies. Such insights will also be of critical importance in the analysis of T cell engraftment in bone marrow recipients. (LB)