CHARACTERIZATION OF MURINE AND HUMAN IG-D-RECEPTORS ON T

小鼠和人类 IG-D-受体在 T 上的表征

• 批准号: 3453615
• 负责人: ASHOK R AMIN
• 金额: $ 11.67万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3453615
• 关键词: T lymphocyte; antibody receptor; antireceptor antibody; binding proteins; carbohydrate receptor; complementary DNA; genetic library; glycoproteins; human tissue; hybridomas; immunoglobulin D; laboratory mouse; molecular cloning; protein purification; protein sequence; receptor binding

Previous observations have established the rapid upregulation by oligomeric or cross-linked, but not monomeric, IgD of receptors for IgD (IgD-R) on a large percentage of murine and human peripheral T cells and helper T cell clones. IgD-R on T cells are also induced by stimulation with anti-CD3, IL-2, IL-4, IFN-gamma, and alternate pathway stimulating agents, such as PMA + ionophore. Many of these reagents, except for IL-2 and alternate pathway stimulators, fail to upregulate IgD-R on T cells from aged mice. In view of the immunoaugmenting properties of IgD-R+ T cells in young, but not in aged mice, these IgD-R appear to stabilize/promote T-B cell interaction, particularly with B cells bearing cross-linked surface IgD. Murine and human helper T hybridoma cells also express IgD-R and release soluble binding factors for IgD (IgD-BF) into their supernatants under the influence of IL-2, IL-4 or crosslinked IgD. Murine IgD-R+ receptors are 40kD molecules that can be specifically precipitated by IgD-Sepharose. These molecules, as well as isolated human IgD-R will be purified and subjected to partial amino acid sequencing. The numbers of IgD-R per cell will be quantitated on normal T cells, IgD-treated T cells and T hybridoma cells. Antibodies to the human IgD-R/BF (LTB9) are available and antibodies to the murine IgD-R/BF are being evaluated for specificity. Cloning of the cDNA of the human and murine IgD-R/BF will be approached using available activated T-cell cDNA libraries. Additional studies will concern the fine specificity of both the murine and human IgD-R: In view of recent findings implicating N-glycans on IgD in its binding to the murine IgD-R, the functional role of these glycans will be investigated. Particular attention will be given to the role of carbohydrate determinants on human IgD in its binding to the human T cell IgD-R.

先前的观察已经确定了低聚物的快速上调