Small Grants for Exploratory Research: Separation of Cells and Biological Macromolecules by Antibody Receptor Coated Magnetic Vesicles and Ferritin Conjugates

探索性研究小额资助：通过抗体受体包被的磁囊和铁蛋白缀合物分离细胞和生物大分子

• 批准号: 9115537
• 负责人: Arijit Bose
• 金额: $ 3.62万
• 依托单位: University of Rhode Island
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115537&HistoricalAwards=false
• 关键词: Small; Grants; Exploratory; Research; Separation

This project explores a new immunomagnetic method for rapidly and efficiently separating cells or biological macromolecules from a mixture. T he method uses vesicles with antibodies Fc-receptors attached on them and with magnetic nanoparticles encapsulated within the aqueous vesicles cores. The vesicles then bind selectively on the antibodies attached to specific cells, which are then separated magnetically. The magnetic vesicles are separated from the cells and can be 'recycled'. A second method using ferritin molecules conjugated with Fc-receptors, rather than vesicles with Fc-receptors, is also explored for comparison. A model system of sheep red blood cells and chicken red blood cells has been successfully separated. The mechanism of binding is investigated by light and electron microscopy. Various separation schemes are explored. The results are potentially applicable to clinical separations - e.g. cancer cells from healthy cells, - biomedical research, and biotechnology.

该项目探索了一种新的免疫磁学方法，可以快速有效地从混合物中分离细胞或生物大分子。该方法使用带有抗体Fc受体的囊泡，并将磁性纳米颗粒包裹在水泡的核心中。然后，这些囊泡选择性地结合到附着在特定细胞上的抗体上，这些抗体随后被磁性分离。磁性囊泡从细胞中分离出来，可以被回收利用。第二种方法是使用铁蛋白分子与Fc受体结合，而不是使用带有Fc受体的囊泡进行比较。成功建立了绵羊红细胞和鸡红细胞的分离模型体系。用光学显微镜和电子显微镜对结合机理进行了研究。探索了各种分离方案。这些结果可能适用于临床分离--例如，从健康细胞中分离癌细胞--生物医学研究和生物技术。