ANALYSIS OF THE SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
ANALYSIS OF THE SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
批准号:
3455231
负责人:
PAUL A GULIG
金额:
$9.77万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-04-01 至 1995-03-31
关键词:
RNA splicing Salmonella infections Salmonella typhimurium active immunization bacterial genetics bacterial proteins gel electrophoresis gene expression genetic manipulation genetic regulation host organism interaction laboratory mouse laboratory rabbit messenger RNA microorganism culture molecular pathology plasmids reporter genes tissue /cell culture virulence
中文摘要
鼠伤寒沙门氏菌和许多其他沙门氏菌是主要的沙门氏菌
美国人类发病和死亡的原因
全世界。 沙门氏菌病主要通过
摄入受污染的肉类、乳制品和家禽产品。 因此,一个
更好地了解沙门氏菌的发病机制应该有助于
通过疫苗开发、改进治疗和预防疾病
疾病管理和改进食品农业方法
生产。
最近,质粒与多种细菌的毒力相关。
沙门氏菌;然而,人们对这一机制或遗传学知之甚少。
质粒介导的毒力。 拟议研究的目标是
因此,要在分子水平上确定
质粒有助于鼠伤寒沙门氏菌的毒力。 第一个具体
目的是鉴定质粒编码的毒力因子和负责的基因
用于将鼠伤寒沙门氏菌传播到小肠以外的口腔
接种的小鼠。 首席研究员已克隆并变异
质粒的毒力基因,这些结果是
继续使用分子质粒,在解剖质粒中进行遗传分析-
介导毒力。 未检测到毒力质粒编码的蛋白质
用S,表示。鼠伤寒杆菌在体外生长,表明毒力
可以调节基因以便仅在动物宿主中表达。
因此,最近分离出Tn5基因。 第二个具体目标是
因此阐明质粒编码的毒力基因是如何受到调节的
通过构建操纵子融合体和分析 mRNA。 毒力
质粒编码的蛋白质及其在鼠伤寒沙门氏菌细胞中的位置将被
作为第三个具体目标进行审查。 这些研究是自然延伸
进一步确定毒力因子及其作用的努力
引发保护性免疫力。
英文摘要
Salmonella typhimurium and many other species of Salmonella are major
causes of morbidity and mortality in humans in the United States and
throughout the world. Salmonellosis is acquired primarily through
ingestion of contaminated meat, dairy, and poultry products. Thus, a
better understanding of pathogenesis of Salmonella should be useful for
prevention of disease through vaccine development, improved therapy and
management of disease, and improved agricultural methods for food
production.
Plasmids have recently been associated with virulence of several species of
Salmonella; however, little is known about the mechanism or the genetics of
plasmid-mediated virulence. The objectives of the proposed research are
therefore to identify at the molecular level mechanism(s) by which the
plasmid contributes to the virulence of S. typhimurium. The first specific
aim is to identify plasmid-encoded virulence factors and genes responsible
for the spread S typhimurium beyond the small intestine of orally
inoculated mice. The principal investigator has cloned and mutated
virulence genes of the plasmid, and these results are the basis for the
continued use of molecular plasmid, genetic analysis in dissecting plasmid-
mediated virulence. Virulence plasmid-encoded proteins were not detectably
expressed by S,. typhimurium growing in vitro, suggesting that virulence
genes may be regulated so as to be expressed only in the animal host.
Therefore, recently isolated Tn5 genes. The second specific aim is
therefore to elucidate how plasmid-encoded virulence genes are regulated
through construction of operon fusions and analysis of mRNA. Virulence
plasmid-encoded proteins and their locations in S typhimurium cells will be
examined as the third specific aim. These studies are a natural extension
of efforts to further characterize virulence factors and their roles in
eliciting protective immunity.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Molecular Pathogenesis of Vibrio vulnificus
-
批准号:6843725
-
项目类别:
-
资助金额:$29.1万
-
财政年份:2003
-
负责人:PAUL A GULIG
-
依托单位:
Molecular Pathogenesis of Vibrio vulnificus
-
批准号:7010054
-
项目类别:
-
资助金额:$28.42万
-
财政年份:2003
-
负责人:PAUL A GULIG
-
依托单位:
Molecular Pathogenesis of Vibrio vulnificus
-
批准号:6675872
-
项目类别:
-
资助金额:$16.31万
-
财政年份:2003
-
负责人:PAUL A GULIG
-
依托单位:
Molecular Pathogenesis of Vibrio vulnificus
-
批准号:6779075
-
项目类别:
-
资助金额:$29.04万
-
财政年份:2003
-
负责人:PAUL A GULIG
-
依托单位:
Molecular Pathogenesis of Vibrio vulnificus
-
批准号:7172578
-
项目类别:
-
资助金额:$27.59万
-
财政年份:2003
-
负责人:PAUL A GULIG
-
依托单位:
ANALYSIS OF THE SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
-
批准号:3455230
-
项目类别:
-
资助金额:$10.28万
-
财政年份:1990
-
负责人:PAUL A GULIG
-
依托单位:
ANALYSIS OF THE SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
-
批准号:3455229
-
项目类别:
-
资助金额:$10.3万
-
财政年份:1990
-
负责人:PAUL A GULIG
-
依托单位:
ANALYSIS OF THE SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
-
批准号:3455228
-
项目类别:
-
资助金额:$10.21万
-
财政年份:1990
-
负责人:PAUL A GULIG
-
依托单位:
SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
-
批准号:2064452
-
项目类别:
-
资助金额:$9.62万
-
财政年份:1990
-
负责人:PAUL A GULIG
-
依托单位:
SALMONELLA TYPHIMURIUM VIRULENCE PLASMID
-
批准号:2064453
-
项目类别:
-
资助金额:$20.24万
-
财政年份:1990
-
负责人:PAUL A GULIG
-
依托单位:
THE VIRULENCE PLASMID OF SALMONELLA TYPHIMURIUM
-
批准号:3029000
-
项目类别:
-
资助金额:$2.5万
-
财政年份:1987
-
负责人:PAUL A GULIG
-
依托单位:
THE VIRULENCE PLASMID OF SALMONELLA TYPHIMURIUM
-
批准号:3028999
-
项目类别:
-
资助金额:$2.0万
-
财政年份:1985
-
负责人:PAUL A GULIG
-
依托单位:
海外基金