EFFECTS OF CHRONIC HIGH FLUORIDE ON ENAMEL DEVELOPMENT

长期高氟对牙釉质发育的影响

• 批准号: 3447169
• 负责人: Pamela K Den Besten
• 金额: $ 5.23万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3447169
• 关键词: ameloblasts; aminoacid; calcium metabolism; dental development; enamel organ; fluoridation; fluorosis; ingested fluoride therapy; tooth enamel; topical fluoride therapy; water supply

Fluorosed dental enamel is caused by the ingestion of above optimal levels of fluoride. Clinically, mild enamel fluorosis appears as white opacities on the teeth, while severe fluorosis results in pitting and loss of surface enamel. These fluorotic teeth are prone to fracture and show abnormal wear patterns which lead to difficult problems in restoration. Fluorosed enamel is characterized by an increase in the content of organic material and a decrease in the mineral. This may be due to a direct effect on the ameloblasts and/or a direct effect on the enamel. The purpose of this proposal is to investigate the effects of the ingestion of chronic high levels of fluoride in drinking water on enamel formation and the mechanisms by which fluorosis occurs. The specific aims include the following: To determine the effects of chronic, high levels of fluoride in the drinking water: (1) on the two classes of proteins in developing enamel; (2) on the cyclic modulation of the maturation ameloblasts as reflected by the staining of the enamel for free and loosely bound calcium; (3) on the morphology of the enamel organ; and (4) to determine the reversibility of the effects of the ingestion of chronic high levels of fluoride in the drinking water on enamel and on the enamel organ. Fluorosed and control enamel proteins will be compared by molecular weight and by amino acid analysis. The modulation of the maturation ameloblasts will be compared histologically using SEM and indirectly by calcein staining of the periodic stripes in maturation enamel. Changes which occur in fluorosed enamel will be compared to the changes which occur in the fluorosed enamel organ. These studies will show when in enamel development high levels of fluoride exert their effect and on which components this effect is manifest, and will lead to further understanding of the mechanisms involved in formation of fluorotic enamel.

含氟牙釉质是由摄入超过最佳水平的牙釉质引起的。 氟化物。临床上，轻度釉质氟中毒表现为白色混浊。 而严重的氟中毒会导致斑点和表面丧失 珐琅。这些患氟牙症的牙齿容易折断，并表现出异常磨损。 这些图案导致了修复中的困难问题。 氟化牙釉质的特点是有机含量增加 材料和矿物的减少。这可能是由于直接的影响。 对成釉细胞和/或对釉质的直接影响。目的 这项建议是为了调查摄入慢性兴奋剂的影响 饮用水中高氟化物对牙釉质形成的影响 氟中毒发生的机制。 具体目标包括以下几点：确定 饮用水中长期、高水平的氟化物：(1)关于两个 牙釉质发育中的蛋白质种类；(2)关于牙釉质的循环调节 成釉细胞的成釉染色 游离和疏松结合的钙：(3)釉器形态； 以及(4)确定摄取的影响的可逆性 长期高氟饮用水中的釉质和 釉质风琴。 氟化的釉质蛋白和对照的釉质蛋白将根据分子量进行比较 并进行氨基酸分析。成釉细胞成熟的调控 将通过扫描电子显微镜进行组织学比较，并通过钙黄绿素进行间接比较 成熟牙釉质中周期性条纹的染色。发生的变化 将氟化牙釉质中的变化与发生在 含氟的釉质器官。 这些研究将表明，在牙釉质发育过程中，高水平的氟化物 发挥它们的影响以及这种影响在哪些组件上明显，以及 将有助于进一步了解形成过程中涉及的机制 氟化牙釉质。