II-GLYCOLIPID BIOSYNTHESIS IN LYMPHOMAS

淋巴瘤中的 II-糖脂生物合成

• 批准号: 3446463
• 负责人: MANJU BASU
• 金额: $ 5.22万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1986-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446463
• 关键词: carcinogenesis; chemical structure; enzyme structure; galactosyltransferases; gas chromatography; glycolipids; glycosphingolipids; lipid structure; lymphoma; mass spectrometry; sugar nucleotide

Immediate objectives of the proposed research are: (1)\Solubilization, separation, and purification of the N-acetylglucosaminyltransferases catalyzing the biosynthesis in vitro of branched Ii-core structure using specific affinity columns from mouse lymphoma. Presence of either of these enzymes in other mouse B-\and T-lymphomas and their possible roles in regulation of Ii-antigen expression on the tumors will be studied. (2)\Using affinity chromatography, betaGalT-4, which catalyzes the biosynthesis in vitro of core nLcOse4Cer, will be separated from betaGlcNAcT and attempts will be made to further purify this betaGalT-4. Exact chemical linkage of the product of betaGalT-4-catalyzed reaction will be determined by permethylation of the product Gal-[14C-Ac]LcOse3Cer. (3)\Different kinetic parameters of these enzymes (betaGlcNAcTs and betaGalT-4) will be studied after further purification of the solubilized enzymes. The major emphasis of this proposal would be to determine the possible relationship between tumorigenicity and expression of Ii-antigen in these mouse B-\and T-lymphomas. (A)

拟议研究的近期目标是： (1)溶解、分离和纯化 N-乙酰葡糖胺转移酶体外催化 使用来自小鼠的特异性亲和柱的分支II-核心结构 淋巴瘤 在其他小鼠B-1中存在这些酶中的任一种， T淋巴瘤及其在Ii抗原表达调节中的作用 将对肿瘤进行研究。 (2)利用亲和层析，β GalT-4，其催化 核心nLcOse体外生物合成4Cer，将与 β GlcNAcT，并尝试进一步纯化该β GalT-4。 β GalT-4催化反应产物的精确化学键将 通过产物Gal-[14C-Ac]LcOse3Cer. (3)这些酶（β GlcNAcTs和β GlcNAcTs）的不同动力学参数 β GalT-4）将在进一步纯化溶解的 内切酶 这项建议的主要重点是确定可能的 肿瘤发生与Ii抗原表达的关系 小鼠B-和T淋巴瘤。 （一）

项目成果

Biosynthesis in vitro of disialosylneolactotetraosylceramide by a solubilized sialyltransferase from embryonic chicken brain.

通过来自胚胎鸡脑的可溶性唾液酸转移酶体外生物合成二唾液酸基新乳四糖神经酰胺。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Higashi,H;Basu,M;Basu,S
• 通讯作者: Basu,S