IMPLANTS ELICITING A FOREIGN BODY REACTION

引起异物反应的植入物

• 批准号: 3448865
• 负责人: HARVEY F SASKEN
• 金额: $ 5.16万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3448865
• 关键词: biological models; biomaterial development /preparation; biomaterial interface interaction; electron microscopy; histochemistry /cytochemistry; immunofluorescence technique; model design /development; polyethylenes; polymers

Must all implants elicit a foreign body reaction? The traditional view is "yes", with a specific cellular and humoral chain of events leading to eventual encapsulation or extrusion. The hypothesis of this proposal is that in each biologic environment the final and permanent stage of the implant-tissue interaction is determined by the intensity, the cellular and extracellular characteristics, and the duration of the initial inflammatory response. And, a more extensive understanding of the initial stage of the response to an implant will allow one to predict, and eventually to control, the ultimate tissue reaction. It is generally considered that a better understanding of the tissue-materials interaction is vital to the development of new materials, implants, and devices. Few investigators with a background in pathology have developed an interest in this area and only a few innovative in-vivo model systems have been proposed to address these questions. The aims of this program are to: 1) Employ four chemically well-characterized polymers. Two of these materials are bioresorbable polyesters derived from Krebs cycle decarboxylic acids with varying bioresorption rates. Two are biodurable materials, polyethylene as a primary reference material, obtained from the NHLBI, and polyethylene terephthalate, purified for subcutaneous implantation. 2) Implant these materials in animals. Two methods of implantation are proposed. The first is a paired subcutaneous implantation in mice. The second (a more innovative method) is parenteral (via tail vein or portal vein) injection of a colloidal suspension with localization in the pulmonary or hepatic parenchyma, respectively. 3) Evaluate the biologic reception of these materials. Standard light and electron microscopy, histochemistry, and morphometry will be employed, as well as the application of new quantative immunofluorescent methods. The analysis protocols as modified and established will have wider application in the field of tissue-materials interaction. 4) Analyze and correlate the biologic reception data. Analysis will be performed to identify features of value in predicting the "outcome" of the tissue material interaction. The third aim, "Analyze the biologic reception of these materials" is the essential concentration of this program. It is in this area that the special expertise gained through anatomic pathologic traning be applied in career development toward addressing the specific (and general) problems in tissue-material interaction analysis.

所有植入物都必须引起异物反应吗？ 传统的观点是 “是”，特定的细胞和体液事件链导致 最终封装或挤出。 该提案的假设是 在每个生物环境中，生物的最后和永久阶段 植入物-组织相互作用由强度、细胞和 细胞外特征和初​​始炎症持续时间 回复。 并且，对初始阶段有更广泛的了解 对植入物的反应将允许人们进行预测，并最终 控制，最终的组织反应。 人们普遍认为，更好地理解 组织与材料的相互作用对于新材料的开发至关重要， 植入物和设备。 具有病理学背景的研究者很少 已经对这一领域产生了兴趣，并且只有少数创新的体内 已经提出模型系统来解决这些问题。 该计划的目标是： 1) 采用四种化学性质良好的聚合物。 其中两个 材料是源自克雷布斯循环的生物可吸收聚酯 具有不同生物吸收速率的脱羧酸。 两种具有生物耐久性 材料，聚乙烯作为主要参考材料，从 NHLBI 和聚对苯二甲酸乙二醇酯，纯化用于皮下注射 植入。 2）将这些材料植入动物体内。 两种植入方法是 建议的。 第一个是在小鼠体内进行配对皮下植入。 这 第二种（更具创新性的方法）是肠外给药（通过尾静脉或门静脉） 静脉）注射胶体悬浮液并定位在 分别为肺实质或肝实质。 3) 评估这些材料的生物接受性。 标准光和 将采用电子显微镜、组织化学和形态测定法， 以及新的定量免疫荧光方法的应用。 这 修改和建立的分析方案将有更广泛的应用 在组织-材料相互作用领域。 4) 分析并关联生物接收数据。 分析将 执行以识别在预测“结果”时有价值的特征 组织材料相互作用。 第三个目标是“分析这些材料的生物接收” 该计划的基本重点。 正是在这个领域， 通过解剖病理学训练获得的特殊专业知识可应用于 职业发展旨在解决特定（和一般）问题 组织-材料相互作用分析。