IDIOTYPES OF RHEUMATOID FACTORS
IDIOTYPES OF RHEUMATOID FACTORS
批准号:
3456752
负责人:
GEORGE F MOXLEY
金额:
$9.29万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-08-01 至 1991-07-31
关键词:
B lymphocyte antigen antibody reaction antigens autoantibody autoimmune disorder cross immunity enzyme linked immunosorbent assay gel electrophoresis human subject immune complex immunoglobulin genes immunoglobulin structure leukocyte activation /transformation molecular pathology monoclonal antibody nucleic acid sequence rheumatoid arthritis rheumatoid factor synovial fluid synthetic nucleic acid synthetic peptide tissue /cell culture
中文摘要
乔治·莫斯利于1976年获得华盛顿大学的医学博士学位,
英文摘要
George Moxley received the MD degree in 1976 from Washington University,
St. Louis. After three years of residency and one year of chief residency
in internal medicine, he has trained as a fellow and now as an assistant
professor of rheumatology, first with a year of intensive clinical exposure
followed by what will be five years of laboratory work with Dr. Shaun
Ruddy. He has developed enzyme-linked immunoassays to identify monoclonal
antibodies which he has produced by somatic cell hybridization and that are
directed toward complement proteins. He has found by radioimmunoassay
striking elevations of C3 anaphylatoxins in synovial fluids of patients
with rheumatoid arthritis (RA) and acute gouty arthritis. The hypothesis
to be examined in this proposal is that patients with RA produce rheumatoid
factors (RFs) with cross-reactive idiotypes which are disease related and
distinct from such immunoglobulins in RF-positive individuals with diseases
other than RA. Substantial progress has been made toward the analysis.
The applicant has developed techniques for preparing single cell
suspensions of lymphocytes from rheumatoid synovial tissue, transforming B
lymphocytes with Epstein-Barr virus, and analyzing culture supernatants for
human immunoglobulin or RFs. He has produced antisera directed toward a
synthetic peptide representing an important part of a monoclonal RF. He
has synthesized oligonucleotides complementary to important parts of RF
light chain genes and used them for hybridization to Southern blots of
patients with rheumatoid arthritis (RA) or normals. The proposed research
will compare immunoglobulin genes among rheumatoid synovium and cells of
RF-positive individuals with diseases other than RA by means of
hybridization with synthetic oligonucleotides; and the primary structure of
immunoglobulins will be compared among rheumatoid synovial fluids or sera
of RF-positive individuals lacking RA by immunoblotting with an antipeptide
antiserum.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Gene Expression Profiles in Osteoarthritis Clinical Variants
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批准号:7739681
-
项目类别:
-
资助金额:$0.79万
-
财政年份:2007
-
负责人:GEORGE F MOXLEY
-
依托单位:
Gene Expression Profiles in Osteoarthritis Clinical Variants
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批准号:7329248
-
项目类别:
-
资助金额:$6.01万
-
财政年份:2007
-
负责人:GEORGE F MOXLEY
-
依托单位:
INFLUENCE OF SEX AND HLA ON RHEUMATOID ARTHRITIS
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批准号:6374336
-
项目类别:
-
资助金额:$9.58万
-
财政年份:1999
-
负责人:GEORGE F MOXLEY
-
依托单位:
INFLUENCE OF SEX AND HLA ON RHEUMATOID ARTHRITIS
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批准号:6632746
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项目类别:
-
资助金额:$10.15万
-
财政年份:1999
-
负责人:GEORGE F MOXLEY
-
依托单位:
INFLUENCE OF SEX AND HLA ON RHEUMATOID ARTHRITIS
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批准号:6512153
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项目类别:
-
资助金额:$9.86万
-
财政年份:1999
-
负责人:GEORGE F MOXLEY
-
依托单位:
INFLUENCE OF SEX AND HLA ON RHEUMATOID ARTHRITIS
-
批准号:6171384
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项目类别:
-
资助金额:$9.48万
-
财政年份:1999
-
负责人:GEORGE F MOXLEY
-
依托单位:
INFLUENCE OF SEX AND HLA ON RHEUMATOID ARTHRITIS
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批准号:2893405
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项目类别:
-
资助金额:$9.2万
-
财政年份:1999
-
负责人:GEORGE F MOXLEY
-
依托单位:
IDIOTYPES OF RHEUMATOID FACTORS
-
批准号:3456756
-
项目类别:
-
资助金额:$9.36万
-
财政年份:1986
-
负责人:GEORGE F MOXLEY
-
依托单位:
IDIOTYPES OF RHEUMATOID FACTORS
-
批准号:3456755
-
项目类别:
-
资助金额:$8.81万
-
财政年份:1986
-
负责人:GEORGE F MOXLEY
-
依托单位:
IDIOTYPES OF RHEUMATOID FACTORS
-
批准号:3456753
-
项目类别:
-
资助金额:$9.66万
-
财政年份:1986
-
负责人:GEORGE F MOXLEY
-
依托单位:
IDIOTYPES OF RHEUMATOID FACTORS
-
批准号:3456754
-
项目类别:
-
资助金额:$9.18万
-
财政年份:1986
-
负责人:GEORGE F MOXLEY
-
依托单位:
海外基金