权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Influence of enhanced antigenicity of renal vascular endothelium induced ischemia/reperfusion injury on the antigen antibody reaction in organ transplantation and the study of protective strategy for enhancedantigenicity

肾血管内皮抗原性增强所致缺血/再灌注损伤对器官移植抗原抗体反应的影响及增强抗原性保护策略的研究

基本信息

批准号：
15591668
负责人：
SATOH Shigeru
金额：
$ 1.73万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591668/
关键词：
ischemiea reperfusion vasucularendothelium costimulatory signal CD80 CD86 phenolic compound resveratrol NO 再灌流障害

项目摘要

Several studies suggested that the vascular endothelial cells function as resident antigen presenting cells by presenting not only human leukocyte antigen but also costimulatory adhesionmoleculesB7 and these molecules expression was up-regulated both during acute rejection and after ischemia/reperfusion (I/R) injury. I/R injury may enhance allograft antigenicity in organ transplantation without antigen-antibody reaction. The present research studied the protective effects of resveratrol, a phenolic product anti-oxidant, on renal function and the expression of CD86 in rat kidneys with I/R injury.Wister rats were divided into four groups ; an I/R group, receiving right nephrectomy and 1-hour clamping of the left renalpedicle ; a Vehicle group, I/R plus 10% ethanol (0.1 ml/kg) by intra-peritoneum from day -1 through to 7; a Resveratrol group, I/S plus 4mg/kg resveratrol ; and a Shamgroup. Blood samples were obtained viathetailveinat-1,1,3,and 7days after the operation for the measurement … More of serum creatinine (Scr) levels. The expression of the CD86 protein was examined by immunofluorescence staining and quantitative level of CD86 messenger RNA (mRNA) was by real time reverse transcription polymerase chain reaction in the renal cortex at day 3. The presence of oxidative damage was assessed by determining the level of thiobarbituric acid reactive substance (TBARS) in renal homogenates.Scr levels of the Resveratrol group were significantly lower than those of the I/R and Vehicle groups on days 1 and 3 after the operation. The expression of CD86 protein in the glomerular endothelium was attenuated in the Resveratrol group compared to the I/R or Vehicle group. In the Resveratrol group, the CD86 mRNA levels was significantly lower than that in the I/R or Vehicle group, moreover, it was significantly lowerly about one fifth than that in the Shamgroup.Our results suggested that resveratrol markedly reduces renaldys function and at tenuates the mRNA and protein expression of CD86 following I/R injury. Less

研究表明，血管内皮细胞作为常驻抗原提呈细胞，不仅提呈人类白细胞抗原，而且还提呈共刺激粘附分子B7，在急性排斥反应和缺血/再灌注（I/R）损伤后，这些分子的表达均上调。在无抗原抗体反应的器官移植中，I/R损伤可增强同种异体抗原性。本研究旨在探讨酚类抗氧化剂白藜芦醇（resveratrol）对大鼠肾I/R损伤后肾功能的保护作用及其对CD 86表达的影响。a媒介物组，I/R加10%乙醇（0.1ml/kg），从第-1天到第7天腹膜内; a白藜芦醇组，I/S加4 mg/kg白藜芦醇;和a假手术组。分别于术后1、1、3、7天经尾静脉采血测定 ...更多信息血清肌酐（Scr）水平。免疫荧光染色检测肾皮质CD 86蛋白的表达，真实的时间反转录聚合酶链反应检测肾皮质CD 86 mRNA的表达。通过测定肾匀浆中硫代巴比妥酸反应物质（TBARS）的水平来评估氧化损伤的存在。白藜芦醇组的Scr水平在术后第1天和第3天显著低于I/R组和溶媒组。与I/R或Vehicle组相比，白藜芦醇组中肾小球内皮中的CD 86蛋白表达减弱。白藜芦醇组CD 86 mRNA表达水平明显低于I/R组和Vehicle组，且较Sham组低约1/5，提示白藜芦醇可明显降低I/R损伤后肾功能，并抑制CD 86 mRNA和蛋白表达。少

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury.

酚类化合物白藜芦醇对缺血/再灌注损伤大鼠肾脏肾功能和共刺激粘附分子 CD86 表达的影响。

DOI：
10.1679/aohc.68.41
发表时间：
2005
期刊：
Archives of histology and cytology
影响因子：
0
作者：
M. Saito*;S. Satoh;N. Kojima;H. Tada;Mitsuru Sato;Toshio Suzuki;H. Senoo;T. Habuchi
通讯作者：
T. Habuchi

Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidney with ischemia/reperfusion injury

酚类化合物白藜芦醇对缺血/再灌注损伤大鼠肾脏肾功能及共刺激粘附分子CD86表达的影响