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Influence of enhanced antigenicity of renal vascular endothelium induced ischemia/reperfusion injury on the antigen antibody reaction in organ transplantation and the study of protective strategy for enhancedantigenicity

肾血管内皮抗原性增强所致缺血/再灌注损伤对器官移植抗原抗体反应的影响及增强抗原性保护策略的研究

基本信息

批准号：
15591668
负责人：
SATOH Shigeru
金额：
$ 1.73万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591668/
关键词：
ischemiea reperfusion vasucularendothelium costimulatory signal CD80 CD86 phenolic compound resveratrol NO 再灌流障害

项目摘要

Several studies suggested that the vascular endothelial cells function as resident antigen presenting cells by presenting not only human leukocyte antigen but also costimulatory adhesionmoleculesB7 and these molecules expression was up-regulated both during acute rejection and after ischemia/reperfusion (I/R) injury. I/R injury may enhance allograft antigenicity in organ transplantation without antigen-antibody reaction. The present research studied the protective effects of resveratrol, a phenolic product anti-oxidant, on renal function and the expression of CD86 in rat kidneys with I/R injury.Wister rats were divided into four groups ; an I/R group, receiving right nephrectomy and 1-hour clamping of the left renalpedicle ; a Vehicle group, I/R plus 10% ethanol (0.1 ml/kg) by intra-peritoneum from day -1 through to 7; a Resveratrol group, I/S plus 4mg/kg resveratrol ; and a Shamgroup. Blood samples were obtained viathetailveinat-1,1,3,and 7days after the operation for the measurement … More of serum creatinine (Scr) levels. The expression of the CD86 protein was examined by immunofluorescence staining and quantitative level of CD86 messenger RNA (mRNA) was by real time reverse transcription polymerase chain reaction in the renal cortex at day 3. The presence of oxidative damage was assessed by determining the level of thiobarbituric acid reactive substance (TBARS) in renal homogenates.Scr levels of the Resveratrol group were significantly lower than those of the I/R and Vehicle groups on days 1 and 3 after the operation. The expression of CD86 protein in the glomerular endothelium was attenuated in the Resveratrol group compared to the I/R or Vehicle group. In the Resveratrol group, the CD86 mRNA levels was significantly lower than that in the I/R or Vehicle group, moreover, it was significantly lowerly about one fifth than that in the Shamgroup.Our results suggested that resveratrol markedly reduces renaldys function and at tenuates the mRNA and protein expression of CD86 following I/R injury. Less

几项研究表明，血管内皮细胞作为常驻抗原呈递细胞，不仅呈递人类白细胞抗原，还呈递共刺激粘附分子B7，并且这些分子的表达在急性排斥期间和缺血/再灌注（I/R）损伤后均上调。 I/R损伤可能会增强器官移植中同种异体移植物的抗原性，而无抗原抗体反应。本研究研究了酚类抗氧化剂白藜芦醇对肾功能的保护作用以及缺血再灌注损伤大鼠肾脏中CD86的表达。Wister大鼠分为四组; I/R组，接受右肾切除并夹闭左肾蒂1小时； a 媒介物组，从第-1天到第7天通过腹膜内I/R加10%乙醇(0.1ml/kg)； a白藜芦醇组，I/S加4mg/kg白藜芦醇；和一个假冒团体。术后 1、1、3 和 7 天通过尾静脉采集血样，用于测量血清肌酐 (Scr) 水平。通过免疫荧光染色检查 CD86 蛋白的表达，并通过实时逆转录聚合酶链反应在肾皮质中定量检测第 3 天的 CD86 信使 RNA (mRNA) 水平。通过测定肾匀浆中硫代巴比妥酸反应物质 (TBARS) 的水平来评估氧化损伤的存在。白藜芦醇组的 Scr 水平显着升高。术后第 1 天和第 3 天低于 I/R 组和车辆组。与 I/R 或媒介物组相比，白藜芦醇组肾小球内皮细胞中 CD86 蛋白的表达减弱。在白藜芦醇组中，CD86 mRNA水平显着低于I/R或Vehicle组，而且显着低于Sham组约五分之一。我们的结果表明，白藜芦醇显着降低肾功能不全，并减弱I/R损伤后CD86的mRNA和蛋白表达。较少的

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury.

酚类化合物白藜芦醇对缺血/再灌注损伤大鼠肾脏肾功能和共刺激粘附分子 CD86 表达的影响。

DOI：
10.1679/aohc.68.41
发表时间：
2005
期刊：
Archives of histology and cytology
影响因子：
0
作者：
M. Saito*;S. Satoh;N. Kojima;H. Tada;Mitsuru Sato;Toshio Suzuki;H. Senoo;T. Habuchi
通讯作者：
T. Habuchi

Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidney with ischemia/reperfusion injury

酚类化合物白藜芦醇对缺血/再灌注损伤大鼠肾脏肾功能及共刺激粘附分子CD86表达的影响