Influence of enhanced antigenicity of renal vascular endothelium induced ischemia/reperfusion injury on the antigen antibody reaction in organ transplantation and the study of protective strategy for enhancedantigenicity

肾血管内皮抗原性增强所致缺血/再灌注损伤对器官移植抗原抗体反应的影响及增强抗原性保护策略的研究

基本信息

批准号：
15591668
负责人：
SATOH Shigeru
金额：
$ 1.73万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591668/
关键词：
ischemiea reperfusion vasucularendothelium costimulatory signal CD80 CD86 phenolic compound resveratrol NO 再灌流障害

项目摘要

Several studies suggested that the vascular endothelial cells function as resident antigen presenting cells by presenting not only human leukocyte antigen but also costimulatory adhesionmoleculesB7 and these molecules expression was up-regulated both during acute rejection and after ischemia/reperfusion (I/R) injury. I/R injury may enhance allograft antigenicity in organ transplantation without antigen-antibody reaction. The present research studied the protective effects of resveratrol, a phenolic product anti-oxidant, on renal function and the expression of CD86 in rat kidneys with I/R injury.Wister rats were divided into four groups ; an I/R group, receiving right nephrectomy and 1-hour clamping of the left renalpedicle ; a Vehicle group, I/R plus 10% ethanol (0.1 ml/kg) by intra-peritoneum from day -1 through to 7; a Resveratrol group, I/S plus 4mg/kg resveratrol ; and a Shamgroup. Blood samples were obtained viathetailveinat-1,1,3,and 7days after the operation for the measurement … More of serum creatinine (Scr) levels. The expression of the CD86 protein was examined by immunofluorescence staining and quantitative level of CD86 messenger RNA (mRNA) was by real time reverse transcription polymerase chain reaction in the renal cortex at day 3. The presence of oxidative damage was assessed by determining the level of thiobarbituric acid reactive substance (TBARS) in renal homogenates.Scr levels of the Resveratrol group were significantly lower than those of the I/R and Vehicle groups on days 1 and 3 after the operation. The expression of CD86 protein in the glomerular endothelium was attenuated in the Resveratrol group compared to the I/R or Vehicle group. In the Resveratrol group, the CD86 mRNA levels was significantly lower than that in the I/R or Vehicle group, moreover, it was significantly lowerly about one fifth than that in the Shamgroup.Our results suggested that resveratrol markedly reduces renaldys function and at tenuates the mRNA and protein expression of CD86 following I/R injury. Less

几项研究表明，血管内皮细胞可作为居民抗原呈现细胞的作用，不仅通过人类白细胞抗原，而且还表现出共刺激性粘合剂分子B7，并且在急性排斥期间和缺血/再灌注后（I/R）损伤期间，这些分子表达都在上调。 I/R损伤可能会增强器官移植中的同类抗原性，而无需抗原 - 抗体反应。本研究研究了白藜芦醇（一种苯酚产物抗氧化剂）对肾功能和I/R损伤大鼠肾脏中CD86表达的保护作用。将妇女大鼠分为四组。 I/R组，接受右肾切除术和左肾per骨的1小时夹紧；从第1天到7，由腹膜内肌内部的I/R加上10％乙醇（0.1 mL/kg）；白藜芦醇组，I/S加4mg/kg白藜芦醇；和一个shamgroup。通过thetailveinat-1,1,3获得血液样本，手术后的7天进行了测量……更多的血清Crectioninine（SCR）水平。 CD86蛋白的表达通过免疫荧光染色和CD86信使RNA的定量水平（mRNA）是通过实时逆转录聚合酶链在第3天的逆转录聚合酶链反应。手术后第1和第3天，低于I/R和车辆组的组。与I/R或媒介物组相比，白藜芦醇组中CD86蛋白在肾小球内皮中的表达被衰减。在白藜芦醇组中，CD86 mRNA水平明显低于I/R或媒介物组的MRNA水平，此外，它的结果明显低于ShamGroup中的五分之一。较少的