A CCD-BASED, COMPUTER-ASSISTED CYTOPHOTOMETRY SYSTEM

计算机辅助细胞光度测定法是一种用于定量细胞中的 特定染色材料的大小、数量和分布 细胞 当与图像分析技术相结合时， 细胞光度测定法成为生物医学研究的一个非常有力的工具。 目前的细胞光度计相对昂贵，通常速度慢，并且许多 商业票据不具备所需的灵活性， 生物医学研究 在过去的十年中，电荷耦合器件（CCD） 成像技术已经发展。 这些新的固态设备具有 高速度、高灵敏度、低噪音和固定的几何形状， 这项新技术对生物医学研究应用有吸引力， 细胞光度测定法 新的研究者研究奖申请提出了 开发、测试和应用计算机辅助细胞光度测定法的建议 系统 这个新系统将包括一个费尔柴尔德CCD图像相机， 蔡司通用显微镜和数字设备公司LSI 11/23 电脑 将开发计算机程序，用于从 CCD器件，图像分割，图像显示，以及总的计算 光密度、细胞面积和形状特征。 的精密度和准确度 将通过比较CCD获得的结果来进行测量 用蔡司扫描载物台对相同细胞进行测量的相机 细胞光度计使用HIDACQUALITY计算机程序。 系统随后将 适用于临床和基础研究项目，包括： 测试免疫刺激剂对溶酶体酶含量的影响， 组织培养巨噬细胞;酶的快速检测 败血症患者中性粒细胞（PMNs） 婴儿;监测慢性阻塞性肺疾病患者的细胞图像采集 淋巴细胞性白血病;荧光变化的实时检测 研究寄主抗病机制的重要性 细胞内病原体;和测量的方向和速度， 组织培养中白细胞运动。 基于CCD的 计算机辅助细胞光度系统将有利于未来的生物医学 通过提供更低的成本，更快和更通用的仪器进行研究。

Computer-assisted cytophotometry is a technique used to quantitate the size, amount and distribution of specifically-stained material within cells. When combined with image analysis techniques, computer-assisted cytophotometry becomes a very powerful tool for biomedical research. Current cytophotometers are relatively expensive, often slow, and many of the commercial instruments do not have the flexibility required for biomedical research. During the past decade charge-coupled device (CCD) imaging technology has developed. These new solid-state devices feature high speed, high sensitivity, low noise and a fixed geometry that makes this new technology attractive for biomedical research applications using cytophotometry. This New Investigator Research Award application presents a proposal to develop, test and apply a computer-assisted cytophotometry system. This new system will consist of a Fairchild CCD image camera, a Zeiss Universal microscope, and a Digital Equipment Corporation LSI 11/23 computer. Computer programs will be developed for acquisition of data from the CCD device, image segmentation, image display, and calculation of total optical density, cell area, and shape features. Precision and accuracy of the measurements will be made by comparing results obtained with the CCD camera with measurements on the same cells made with a Zeiss scanning stage cytophotometer using the HIDACSYS computer programs. The system will then be applied to both clinical and basic research projects, including: testing the effects of immunostimulants on the lysosomal enzyme content of macrophages cultured in tissue culture; rapid detection of enzyme differences in polymorphonuclear neutrophils (PMNs) from septicemic infants; acquisition of cell images for monitoring patients with chronic lymphocytic leukemia; real-time detection of changes in fluorescence intensity for the study of the mechanisms of host resistance to intracellular pathogens; and measurement of direction and speed of leukocyte movement in tissue culture. Development of a CCD-based computer-assisted cytophotometry system will benefit future biomedical research by providing a lower cost, faster and more versatile instrument.