A CCD-BASED, COMPUTER-ASSISTED CYTOPHOTOMETRY SYSTEM
基于 CCD 的计算机辅助细胞光度测定系统
基本信息
- 批准号:3450322
- 负责人:
- 金额:$ 4.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1984
- 资助国家:美国
- 起止时间:1984-01-01 至 1986-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Computer-assisted cytophotometry is a technique used to quantitate the
size, amount and distribution of specifically-stained material within
cells. When combined with image analysis techniques, computer-assisted
cytophotometry becomes a very powerful tool for biomedical research.
Current cytophotometers are relatively expensive, often slow, and many of
the commercial instruments do not have the flexibility required for
biomedical research. During the past decade charge-coupled device (CCD)
imaging technology has developed. These new solid-state devices feature
high speed, high sensitivity, low noise and a fixed geometry that makes
this new technology attractive for biomedical research applications using
cytophotometry. This New Investigator Research Award application presents
a proposal to develop, test and apply a computer-assisted cytophotometry
system. This new system will consist of a Fairchild CCD image camera, a
Zeiss Universal microscope, and a Digital Equipment Corporation LSI 11/23
computer. Computer programs will be developed for acquisition of data from
the CCD device, image segmentation, image display, and calculation of total
optical density, cell area, and shape features. Precision and accuracy of
the measurements will be made by comparing results obtained with the CCD
camera with measurements on the same cells made with a Zeiss scanning stage
cytophotometer using the HIDACSYS computer programs. The system will then
be applied to both clinical and basic research projects, including:
testing the effects of immunostimulants on the lysosomal enzyme content of
macrophages cultured in tissue culture; rapid detection of enzyme
differences in polymorphonuclear neutrophils (PMNs) from septicemic
infants; acquisition of cell images for monitoring patients with chronic
lymphocytic leukemia; real-time detection of changes in fluorescence
intensity for the study of the mechanisms of host resistance to
intracellular pathogens; and measurement of direction and speed of
leukocyte movement in tissue culture. Development of a CCD-based
computer-assisted cytophotometry system will benefit future biomedical
research by providing a lower cost, faster and more versatile instrument.
计算机辅助细胞光度法是一种用于定量测定细胞中细胞数量的技术
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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RICHARD M DONOVAN其他文献
RICHARD M DONOVAN的其他文献
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{{ truncateString('RICHARD M DONOVAN', 18)}}的其他基金
DEVELOPMENT AND EVALUATION OF A TIME-RESOLVED MICROSCOPE
时间分辨显微镜的开发和评估
- 批准号:
3431697 - 财政年份:1989
- 资助金额:
$ 4.76万 - 项目类别:
METHODS TO DETECT HIV AND RELATED RETROVIRUSES
检测艾滋病毒和相关逆转录病毒的方法
- 批准号:
3596147 - 财政年份:1987
- 资助金额:
$ 4.76万 - 项目类别:
METHODS TO DETECT HIV AND RELATED RETROVIRUSES
检测艾滋病毒和相关逆转录病毒的方法
- 批准号:
3596144 - 财政年份:1987
- 资助金额:
$ 4.76万 - 项目类别:
METHODS TO DETECT HIV GENETIC RELATED RETROVIRUSES
检测 HIV 基因相关逆转录病毒的方法
- 批准号:
3596148 - 财政年份:1987
- 资助金额:
$ 4.76万 - 项目类别:
METHODS TO DETECT HIV GENETIC RELATED RETROVIRUSES
检测 HIV 基因相关逆转录病毒的方法
- 批准号:
3596150 - 财政年份:1987
- 资助金额:
$ 4.76万 - 项目类别:
METHODS TO DETECT HIV GENETIC RELATED RETROVIRUSES
检测 HIV 基因相关逆转录病毒的方法
- 批准号:
3596145 - 财政年份:1987
- 资助金额:
$ 4.76万 - 项目类别:
A CCD-BASED, COMPUTER-ASSISTED CYTOPHOTOMETRY SYSTEM
基于 CCD 的计算机辅助细胞光度测定系统
- 批准号:
3450323 - 财政年份:1984
- 资助金额:
$ 4.76万 - 项目类别: