RAPID PRESSURE RISE: VASCULAR RESISTANCE AND STEREOLOGY

压力快速上升：血管阻力和体视学

• 批准号: 3448637
• 负责人: JAMES G WALMSLEY
• 金额: $ 5.26万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3448637
• 关键词: blood pressure; cellular pathology; histology; hypertension; microangiography; peripheral blood vessel; scanning electron microscopy; vascular resistance

This is a proposal to study the time course of changes in rabbit ear vascular resistance and structure after partial abdominal aortic constriction (PAAC). The specific aims include: (1) determination of the in vitro resistancae-flow-pressure-characteristics of the ear vascular bed from normotensive and hypertensive rabbits, (2) quantitation of the morphological parameters which are known to effect the vascular resistance directly i.e. the number, length and radius of the different sized vessels, (3) description in quantitative stereological terms of the components in the arterial wall which are responsible for the active and passive characteristics of the various sized arteries, (4) measurement of the size, orientation and number of medial smooth muscle cells using established methods of stereology, (5) quantitation of muscle cell and endothelial ultrastructure with regard to proportional composition and organization of the contractile apparatus, organelles and membranes. These studies will be pursued using a variety of approaches and techniques: (1) post-surgical constriction of the aorta to initiate time course studies, (2) vertical tube determination of flow-pressure curves for the ear vasculature with supporting constant flow and pressure measurements, (3) resistance measurement in response to sympathetic agonists and nerve stimulation, (4) pressure fixation under functionally defined conditions, (5) microangiographic and scanning electron microscopic determination of vascular geometry, (6) plastic embedding of tissues for light and transmission electron microscopy, (7) morphometry and stereology with the image analysis graphics computer system. In general, these studies should further our knowledge of correlation between basic functional and structural changes in developing hypertension. Specifically, the proposed study will investigate one intact vascular bed in detail to determine how changes in pressure and vascular resistance are related to morphometry of the vascular network and stereology of the vascular wall.

这是一个研究兔耳变化时间过程的建议