RAPID PRESSURE RISE: VASCULAR RESISTANCE AND STEREOLOGY

这是一个研究兔耳变化的时间过程的建议 部分腹主动脉后血管阻力和结构 收缩（PAAC）。 具体目标包括：（1）确定 耳血管床的体外阻力-流量-压力-特性 从正常血压和高血压兔，（2）定量 已知影响血管阻力的形态参数 直接地，即不同尺寸血管的数量、长度和半径， (3)用定量体视学术语描述 动脉壁负责主动和被动 各种尺寸的动脉的特性，（4）尺寸的测量， 中膜平滑肌细胞的方向和数量 体视学方法;（5）肌细胞和内皮细胞的定量 超微结构方面的比例组成和组织 收缩器、细胞器和细胞膜。 这些研究报告将 采用各种方法和技术进行：（1）手术后 收缩主动脉以启动时程研究，（2）垂直 耳脉管系统的流量-压力曲线的管测定， 支持恒定流量和压力测量，（3）阻力 测量对交感神经激动剂和神经刺激的反应，（4） 功能规定条件下的压力固定，（5） 微血管造影和扫描电子显微镜测定 血管几何形状，（6）组织的塑料包埋， 透射电子显微镜，（7）形态测定和体视学与 图像分析图形计算机系统。 总的来说，这些研究应该 进一步了解基本功能和 结构变化发展高血压。 具体而言，建议 研究将详细调查一个完整的血管床，以确定如何 压力和血管阻力的变化与 血管网和血管壁的体视学。

This is a proposal to study the time course of changes in rabbit ear vascular resistance and structure after partial abdominal aortic constriction (PAAC). The specific aims include: (1) determination of the in vitro resistancae-flow-pressure-characteristics of the ear vascular bed from normotensive and hypertensive rabbits, (2) quantitation of the morphological parameters which are known to effect the vascular resistance directly i.e. the number, length and radius of the different sized vessels, (3) description in quantitative stereological terms of the components in the arterial wall which are responsible for the active and passive characteristics of the various sized arteries, (4) measurement of the size, orientation and number of medial smooth muscle cells using established methods of stereology, (5) quantitation of muscle cell and endothelial ultrastructure with regard to proportional composition and organization of the contractile apparatus, organelles and membranes. These studies will be pursued using a variety of approaches and techniques: (1) post-surgical constriction of the aorta to initiate time course studies, (2) vertical tube determination of flow-pressure curves for the ear vasculature with supporting constant flow and pressure measurements, (3) resistance measurement in response to sympathetic agonists and nerve stimulation, (4) pressure fixation under functionally defined conditions, (5) microangiographic and scanning electron microscopic determination of vascular geometry, (6) plastic embedding of tissues for light and transmission electron microscopy, (7) morphometry and stereology with the image analysis graphics computer system. In general, these studies should further our knowledge of correlation between basic functional and structural changes in developing hypertension. Specifically, the proposed study will investigate one intact vascular bed in detail to determine how changes in pressure and vascular resistance are related to morphometry of the vascular network and stereology of the vascular wall.